钙离子结合蛋白S100A4在胰腺癌组织中的表达及其意义

目的探讨胰腺癌组织中钙离子结合蛋白S100A4的表达及其临床意义。方法应用免疫组化EliVisionTMPlus法检测70例手术切除的原发洼胰腺癌组织及15例癌旁非肿瘤胰腺组织中S100A4蛋白的表达情况，比较S100A4表达与临床病理指标的关系。结果70例标本中S100A4阳性率为74．3％（52／70），而癌旁非肿瘤胰腺组织中未见表达，差异有统计学意义（χ2=28．70，P〈0．05）；S100A4表达与性别、年龄及肿瘤部位无关（P〉0.05），与肿瘤大小、分化程度、TNM分期、淋巴结转移、生存期显著相关（P〈0.05）。S100A4表达阳性组与阴性组之间无瘤生存率（χ2=13．12，P〈0．05）及总体生存率（χ2=20.64，P〈0.05）差异均有统计学意义。结论S100A4蛋白的高表达与胰腺癌的临床病理指标密切相关，其高表达提示患者预后不良。

Expression of S100A4 in pancreatic carcinoma and its significance

Objective To investigate the expression of calcium-binding protein S100A4 in pancreatic carcinoma and its clinical significannce. Methods Immunohistochemistry EliVisionTM Plus method was used to examine the expression of S100A4 in 70 surgical specimens of primary pancreatic carcinoma and 15 patients with noncarcinoma pancreatic tissues. The correlation between the expression of S100A4 and the clinicopathological parameters was analyzed. Results S100A4 was positive in 52 （52/70, 74. 3% ）specimens of primary pancreatic carcinoma according to immunohistochemistry detection. No expression of S100A4 in adjacent noncarcinoma pancreatic tissues was detected. The expression of S100A4 did not correlated with gender, age or tumor site while it was significantly correlated with tumor size, grade of differentiation, TNM stages, lymph node metastasis and survival. Disease-free survival and overall survival of the negative group were significantly longer than the positive group. The difference had statistical significance. Conclusion The study shows that over expression of S100A4 protein is closely related with clinicopathological parameters of pancreatic cancer patients, indicating poor prognosis for pancreatic cancer patients.