ATMFS对犬骨盆弓状线骨折愈合及骨钙蛋白和骨唾液酸蛋白表达的影响

目的探索骨盆髋臼三维记忆内固定系统(acetabular tridimensional memory fixation system,ATMFS)对犬弓状线骨折愈合及骨钙蛋白和骨唾液酸蛋白表达的影响。方法选用15只成年杂种家犬,双侧髋臼臼顶上方1.5cm处横形截骨,分别采用ATMFS前柱固定器和6孔重建钢板内固定,于术后2、4、6、8、12周行影像学检查、大体观察、骨钙蛋白及骨唾液酸蛋白原位杂交、骨钙蛋白实时定量PCR(RT-PCR)检测,评价骨盆弓状线的骨愈合特征及骨钙蛋白和骨唾液酸蛋白的表达特征。结果 ATMFS侧骨折端无凌乱骨痂,骨折愈合时间明显快于钢板侧。双侧骨折端组织中骨钙蛋白和骨唾液酸蛋白表达与正常比均明显增强。术后4～8周,ATMFS侧骨钙蛋白和骨唾液酸蛋白表达量明显大于钢板侧,两侧比较具有显著性差异(P0.05),其中术后6周双侧差异最为显著(P0.01)。结论 ATMFS固定后于骨折端产生的持续顺应生理力线的压应力能够刺激骨钙蛋白及骨唾液酸蛋白的表达,从而促进骨折早期愈合。

The effects of ATMFS on the bone healing of iliopectineal crest fracture and the expression of osteocalcin and bone sialoprotein

Objective　To explore the effects of acetabular tridimensional memory fixation system（ATMFS） on the bone healing and the expression of osteocalcin and bone sialoprotein of canine iliopectineal crest fracture models.　Methods　15 adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5 cm above the dome of acetabulum，which were fixed with ATMFS and steel plate respectively. The animals were examined at 2，4，6，8 and 12 weeks after operation by X-ray and macropathological observation. The expression of osteocalcin and bone sialoprotein were investigated by in situ hybridization, histological image analysis and real-time PCR respectively.　Results　There was no disordered bony callus appeared in ATMFS fixed bone, with the shorter healing time of it compared to that of steel plate fixation. The more strong expression of osteocalcin and bone sialoprotein can be found in fixation groups compared to that of the control, furthermore, significantly strong expression of them appeared in ATMFS group than that of steel plate group（P<0.05）, especially at the 6 weeks（P<0.01）after the operation.　Conclusions　ATMFS is valuable for the production of osteocalcin and bone sialoprotein during the process of bone healing by generating persistent compressive stress.