白细胞介素-1β和白细胞介素-6对妊娠相关血浆蛋白-A表达的影响

目的探讨炎性因子白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)对人冠状动脉平滑肌细胞(human coronary artery smooth muscle cell,HCASMC)表达妊娠相关血浆蛋白-A(pregnancy-associated plasma pro-tein-A,PAPP-A)的影响。方法应用20μg/L的IL-1β、10μg/L的IL-6各自刺激HCASMC,共同培养0、2、4、8、243、6 h后收集细胞。应用不同浓度的IL-1β(0、5、20、40μg/L)I、L-6(0、5、10、50μg/L)刺激HCASMC,共同培养6 h后收集细胞。应用实时定量聚合酶链反应的方法检测细胞内PAPP-A基因的表达量。结果在同剂量IL-1βI、L-6刺激下,PAPP-A的表达量在2 h时就开始发生上调,8 h达高峰,而后开始下降;在不同剂量IL-1βI、L-6刺激下,PAPP-A的表达量在实验剂量范围内随着剂量的加大呈上升趋势(IL-1β:r=0.972,P=0.000;IL-6:r=0.941,P=0.000)。结论炎性因子IL-1βI、L-6能促进HCASMC中斑块稳定相关标记物PAPP-A的表达,可能是炎症在急性冠状动脉综合征发生发展中的重要作用机制之一。

The influence of IL-1β and IL-6 on pregnancy-associated plasma protein-A gene expression

Objective To study the effect of IL-1β and IL-6 on pregnancy-associated plasma protein-A(PAPP-A) gene expression in human coronary artery smooth muscle cells.Methods Human coronary artery smooth muscle cells were stimulated with IL-1β(20 μg/L) or IL-6(10 μg/L).RNA was isolated and culture medium was collected after 0,2,4,8,24 and 36 h.Then the human coronary artery smooth muscle cells were stimulated with IL1β(0,5,20,40 μg/L) or IL-6(0,5,10,50 μg/L).RNA was isolated and culture medium was collected after 6 h.Real-time PCR was performed on RNA to detect PAPP-A gene expression.Results At the same concentration of IL-1β and IL-6,gene expression was up-regulated at 2 hours,and reached the peak at 8 hours,then began to decrease.At different concentrations of IL-1β and IL-6,the gene expression was up-regulated with increasing the dose of IL-1β and IL-6(IL-1β:r=0.972,P=0.000;IL-6:r=0.941,P=0.000).Conclusion It is suggested that IL-1β and IL-6 can promote PAPP-A gene expression in human coronary artery smooth muscle cells,this may be one of the mechanisms of inflammation effect in development of acute coronary syndrome.