| 红豆杉细胞提取物抑制肿瘤细胞增殖及诱导凋亡的实验研究 |
| |
| 引用本文： | 鲁翠涛,赵应征,梅兴国.红豆杉细胞提取物抑制肿瘤细胞增殖及诱导凋亡的实验研究[J].医学教育探索,2003(11):1013-1015. |
| |
| 作者姓名： | 鲁翠涛 赵应征 梅兴国 |
| |
| 作者单位： | [1]华中科技大学生命科学与技术学院，湖北武汉430074 [2]军事医学科学院毒物药物研究所，北京100850 |
| |
| 基金项目： | “九五”国家攻关项目 (96- C0 2 - 0 3- 0 1 ) |
| |
| 摘 要： | 目的 研究红豆杉细胞提取物对肝癌细胞SMMC-7721的生长抑制作用及其机制。方法 运用台盼蓝拒染法测定肿瘤细胞生长曲线，流式细胞仪和Giemsa染色分析肿瘤细胞周期和细胞凋亡。结果 红豆杉细胞二氯甲烷提取物对肝癌细胞SMMC-7721的生长有明显的抑制作用，流式细胞仪分析发现G2／M期肿瘤细胞数量增多，经Giemsa染色发现肿瘤细胞有凋亡发生。结论 红豆杉细胞提取物可以通过诱导细胞发生凋亡而抑制肝癌细胞SMMC-7721的生长。
|
| 关 键 词： | 红豆杉细胞 SMMC-7721 细胞增殖 细胞凋亡 |
Extract from cultured cells of Taxus chinensis on proliferation inhibition and apoptosis induction of tumor cells |
| |
| LU Cui tao,ZHAO Ying zheng,MEI Xing guo.Extract from cultured cells of Taxus chinensis on proliferation inhibition and apoptosis induction of tumor cells[J].Researches in Medical Education,2003(11):1013-1015. |
| |
| Authors: | LU Cui tao ZHAO Ying zheng MEI Xing guo |
| |
| Abstract: | Object To study the inhibitory effect and mechanism of extract from cultured cell of Taxus chinensis (Pilg.) Rehd. on hepatocarcinoma cell line SMMC 7721. Methods Trypan blue stain assay was used to determine tumor cell growth curve, flow cytometry and Giemsa staining were used to analyze cell cycle and cell apoptosis. Results Inhibitory effect happened on tumor cell line SMMC 7721, G 2/M stage of tumor cells increased with the addition of T. chinensis cell extract by carrene and apoptosis happened. Conclusion The extracts from cultured cell of T. chinensis have inhibitory effect on SMMC 7721 cells and can further induce cell apoptosis. |
| |
| Keywords: | cell of Taxus chinensis (Pilg ) Rehd SMMC 7721 cell proliferation cell apoptosis |
|
| 点击此处可从《医学教育探索》浏览原始摘要信息 |
| 点击此处可从《医学教育探索》下载免费的PDF全文 |
