| 双向电泳分析叶酸对神经干细胞蛋白表达的影响 |
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| 引用本文： | 刘欢,黄国伟,何冰,杨阳,赵琳.双向电泳分析叶酸对神经干细胞蛋白表达的影响[J].天津医药,2011,39(7):615-617. |
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| 作者姓名： | 刘欢 黄国伟 何冰 杨阳 赵琳 |
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| 作者单位： | 1. 天津医科大学公共卫生学院,300070
2. 武警医学院天津市职业与环境危害生物标志物重点实验室 |
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| 基金项目： | 国家自然科学基金资助项目(项目编号:81072289,30771797) |
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| 摘 要： | 目的:探讨叶酸缺乏对胚胎大鼠神经干细胞(NSCs)蛋白质表达的影响。方法:采用无血清体外细胞培养方法,培养胚胎大鼠NSCs并进行鉴定,采用叶酸缺乏培养基培养NSCs,根据叶酸添加终浓度将细胞分为对照(Con?trol)组、叶酸缺乏(Folate-D)组、叶酸补充(Folate-L)组,各组叶酸终浓度分别为4、0.6、8mg/L。应用双向电泳(2-DE)技术分析各组蛋白质表达的差异。结果:建立了稳定可重复的NSCs2-DE图谱,与Control组相比,Folate-D组2个蛋白点表达量降低,4个蛋白点表达量升高;Folate-L组2个蛋白点表达量降低,1个蛋白点表达量升高。结论:叶酸缺乏或补充叶酸影响NSCs蛋白表达,差异蛋白分离为进一步研究叶酸对NSCs增殖分化调控机制奠定了基础。
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| 关 键 词： | 电泳 凝胶 双向 叶酸 干细胞 蛋白质组学 大鼠 Sprague-Dawley |
Effect of Folate on Protein Expression in Neural Stem Cells Analyzed by Two-Dimensional Gel Electrophoresis |
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| LIU Huan,HUANG Guowei,HE Bing,YANG Yang,ZHAO Lin.Effect of Folate on Protein Expression in Neural Stem Cells Analyzed by Two-Dimensional Gel Electrophoresis[J].Tianjin Medical Journal,2011,39(7):615-617. |
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| Authors: | LIU Huan HUANG Guowei HE Bing YANG Yang ZHAO Lin |
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| Institution: | LIU Huan,HUANG Guowei,HE Bing,YANG Yang,ZHAO Lin School of Public Health,Tianjin Medical University,Tianjin 300070,China |
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| Abstract: | Objective:To analyze the effect of folate deficiency on protein expression of embryonic rat neural stem cells(NSCs).Methods:Embryonic rat NSCs were cultured in serum-free suspension culture and were identified.Individual cultures were assigned to one of 3 treatment groups according to the concentration of folate in the medium:control group,folate deficiency group(Folate-D) and folate supplementation group(Folate-L).The final folate concentrations were 4,0.6 and 8 mg/L respectively.Protein expressions were d... |
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| Keywords: | electrophoresis gel two-dimensional folic acid stem cells proteomics rats Sprague-Dawley |
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