mitoK(ATP)介导硫化氢预处理延迟相的心肌保护效应

目的:研究线粒体ATP敏感性钾通道mitoK(ATP)]激活在硫化氢预处理延迟相对大鼠心肌细胞的保护效应。方法:将32只健康成年雄性SD大鼠随机分成假手术组(Sham组)、缺血再灌注组(IR组)、硫化氢预处理组(HS组)和5-羟葵酸(5-HD)+硫化氢预处理组(HD组),每组8只。Sham组仅穿线但不阻断左冠状动脉前降支(LAD);IR组结扎LAD30min,松解后再灌注120min;HS组静脉注射硫化氢供体NaHS50μg/kg,24h后同IR组处理;HD组于结扎前15min静脉注射5-HD5mg/kg,其他同HS组处理。检测心室面积、心肌缺血面积和梗死面积,计算缺血和梗死面积百分比;电镜下观察心肌细胞超微结构。结果:3组缺血面积百分比差别无统计学意义(P>0.05);HS组心肌梗死面积百分比低于IR组和HD组(P<0.01或P<0.05),HD组与IR组相比差异无统计学意义(P>0.05)。心肌细胞超微结构损伤程度HS组较IR组明显减轻,而HD组与IR组差异不大。结论:硫化氢预处理延迟相可保护缺血再灌注损伤的心肌,mitoK(ATP)介导了硫化氢预处理延迟相的心肌保护通路。

mitoK(ATP)Mediates the Protective Effect of Late Phase of Hydrogen Sulfide Preconditioning on Myocardial Cells

Objective:To investigate the protective effect of hydrogen sulfide induced late phase preconditioning on rat myocardial cells with mitochondrial ATP sensitive potassium channel mitoK(ATP)] activation.Methods:Thirty-two adult male SD rats were randomly divided into 4 groups including Sham group,ischemia and reperfusion(IR) group,hydrogen sulfide treatment(HS) group and 5-hydroxy decanoate(5HD) + HD group(n= 8 for each group).In Sham group,rats were threaded left anterior descending(LAD) coronary artery,but ...