参麦注射液对大鼠心肌缺血及NO形成的影响

目的　探讨参麦注射液 (SMI)预处理可能对大鼠心肌缺血及NO形成的影响。方法　实验大鼠随机机分为对照组 (生理盐水 )、模型组 (单纯心肌缺血 )、处理组 1(心肌缺血 低剂量SMI)、处理组 2 (心肌缺血 高剂量SMI) ,应用异丙肾上腺素建立大鼠心肌缺血模型 ,以心电图ST段偏移值作为心肌缺血损伤指标 ,心肌缺血 4 0min时 ,用硝酸还原酶法测定大鼠血清和心肌组织的NO。结果　与对照组比较 ,模型组各时间点的心电图ST段均显著抬高 ,并于缺血 2 0min时达到高峰 (P <0 0 0 1) ,血清和心肌组织的NO含量均显著降低 (P <0 0 1) ;与模型组比较 ,两个处理组于缺血 2 0、30、4 0min时的心电图ST段抬高幅度显著降低 (P <0 0 5 ) ,血清和心肌组织的NO含量均显著升高 (P <0 0 5 ) ;处理组 1和处理组 2比较 ,血清和心肌组织的NO含量及心电图ST段抬高幅度差异均无显著性 (P >0 0 5 )。结论　参麦注射液可能是通过升高NO水平达到抗心肌缺血的作用

Effect of shenmai injection on nitric oxide in rats with experimental myocardial ischemia

Objective To study the effect of shenmai injection on nitric oxide in rats with experimental myocardial ischemia. Methods Rats were randomly divided into four groups: control group, ischemia group, shenmai injection group 1 and shenmai injection group 2. Rat models of acute myocardial ischemia were created by isoprenaline, and the lift of ST segment in ECG was used as the index of myocardial ischemia. The NO contents of serum and myocardium in rats with acute myocardial ischemia were measured 40 minutes after ischemia. Results Compared with control group, the concentration of NO in the serum and myocardium of ischemia group was significantly lowered(P<0.01). ST segment of ECG in ischemia group was significantly elevated and the lift peak of ST segment occur 20 min after myocardial ischemia(P<0.001). Compared with the ischemia group, the concentration of NO in the serum and myocardium of SMI group 1 and SMI group 2 significantly increased(P<0.05). The lift of ST segment were significantly reduced in SMI group 1 and SMI group 2(P<0.05). Compared with SMI group 1, the concentration of NO in the serum and myocardium and the lift of ST segment were not statistically different in SMI group 2(P>0.05). Conclusion Shenmai injection can increase the content of NO and improving myocardial ischemia in rats.