| HER-2 RNA干扰对HER-2阳性乳腺癌化疗敏感性的研究 |
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| 引用本文： | 胡孝渠,叶志强,郭贵龙,尤捷. HER-2 RNA干扰对HER-2阳性乳腺癌化疗敏感性的研究[J]. 中华肿瘤防治杂志, 2010, 17(1) |
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| 作者姓名： | 胡孝渠 叶志强 郭贵龙 尤捷 |
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| 作者单位： | 温州医学院附属第一医院肿瘤外科,浙江,温州,325000 |
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| 基金项目： | 浙江省自然科学基金(Y207301); 温州医学院一附院优秀硕博士预知科研基金(2007SB02) |
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| 摘 要： | 目的:探讨HER-2 RNA干扰(RNAi)对HER-2阳性乳腺癌细胞的化疗敏感性的影响.方法:构建能够表达HER-2siRNA的质粒栽体,利用载体转染HER-2阳性乳腺癌细胞SKBR-3,RT-PCR与蛋白质印迹法检测HER-2 mRNA与蛋白表达,受转染的细胞与不同浓度的化疗药物表柔比星共培养,MTT法检测细胞活性.结果:SKBR-3细胞HER-2基因受到RNAi后,HER-2 mRNA及蛋白表达分别下降约60%与55%,细胞活性出现明显下降,肿瘤细胞对表柔比星的半数抑制浓度(IC50)为0.25 μg/μL,相较对照组而言,化疗敏感性提高明显.结论:乳腺癌HER-2是理想的RNA干扰治疗靶点,靶向HER-2的RNAi可以抑制肿瘤细胞生长,而且能够增加肿瘤细胞化疗敏感性.
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| 关 键 词： | 乳腺肿瘤 RNA干扰 抗肿瘤药 |
Effects of HER-2 RNA interference on HER-2-positive breast cancer cell sensitivity to chemotherapeutic agent |
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| HU+Xiao-qu,YE+Zhi-qiang,GUO+Gui-long,YOU+Jie+Department+of+Surgery+Oncology,First+Affiliated+Hospital+of+Wenzhou+Medical+College,Wenzhou+,P.R.China. Effects of HER-2 RNA interference on HER-2-positive breast cancer cell sensitivity to chemotherapeutic agent[J]. Chinese Journal of Cancer Prevention and Treatment, 2010, 17(1) |
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| Authors: | HU+Xiao-qu YE+Zhi-qiang GUO+Gui-long YOU+Jie+Department+of+Surgery+Oncology First+Affiliated+Hospital+of+Wenzhou+Medical+College Wenzhou+ P.R.China |
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| Affiliation: | HU+Xiao-qu,YE+Zhi-qiang,GUO+Gui-long,YOU+Jie+Department+of+Surgery+Oncology,First+Affiliated+Hospital+of+Wenzhou+Medical+College,Wenzhou+325000,P.R.China |
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| Abstract: | OBJECTIVE:To study the effects HER-2 gene RNA interference (RNAi) on the chemo-sensitivity of HER-2-positive breast cancer cell. METHODS:A plasmid vector capable of mediating HER-2 RNAi was constructed,and HER-2 positive breast cancer cell line SKBR-3 was transfected with constructed vector. The expression of HER-2 mRNA and protein was analyzed by RT-PCR and Western blotting; Also,transfected cells were co-cultured with different concentrations of epirubicin and the growth of SKBR-3 cells was analyzed by MT... |
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| Keywords: | breast neoplasms RNA interference antineoplastic agents |
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