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| RNAi沉默STAT3基因对结肠癌HCT116细胞侵袭的调控 | |
| 引用本文： | 范钰,张尤历,张宇川,吴莺,许则丰,万毅刚. RNAi沉默STAT3基因对结肠癌HCT116细胞侵袭的调控[J]. 中国临床医学, 2007, 14(4): 512-514 |
| 作者姓名： | 范钰 张尤历 张宇川 吴莺 许则丰 万毅刚 |
| 作者单位： | 1. 江苏大学附属医院消化科,江苏镇江,212001 2. 浙江大学肿瘤研究所,浙江杭州,310009 3. 南京大学医学院附属鼓楼医院中医科,江苏南京,210008 |
| 摘 要： | 目的:观察RNA干扰沉默STAT3基因对结肠癌细胞侵袭的影响,并探讨其可能机制。方法:应用STAT3小干扰RNA(small interfering RNA,siRNA)转染处理人结肠癌细胞系HCT116后,采用RT-PCR检测STAT3 mRNA表达,软琼脂集落培养试验检测锚着不依赖性增殖,Boyden小室模型试验检测癌细胞的侵袭性,琼脂糖凝胶电泳和TUNEL检测癌细胞失巢凋亡。结果:STAT3 siRNA可有效抑制结肠癌细胞集落生长和侵袭能力,且与浓度相关。琼脂糖凝胶电泳和TUNEL结果显示,STAT3 siRNA转染可诱导结肠癌细胞失巢凋亡,且与浓度相关。结论:STAT3 siRNA可抑制结肠癌细胞的恶性侵袭,其机制与诱导失巢凋亡有关。 |
| 关 键 词： | 结肠肿瘤 信号转导子和转录激活子3 RNA干扰 小干扰RNA 侵袭 失巢凋亡 |
Regulation of STAT3 siRNA on Invasion of Colon Cancer Cell Line HCT116 |
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| FAN Yu,ZHANG Youli,ZHANG Yuchuan,WU Ying,XU Zhefeng,WAN Yigang. Regulation of STAT3 siRNA on Invasion of Colon Cancer Cell Line HCT116[J]. Chinese Journal Of Clinical Medicine, 2007, 14(4): 512-514 | |
| Authors: | FAN Yu ZHANG Youli ZHANG Yuchuan WU Ying XU Zhefeng WAN Yigang |
| Affiliation: | Deparment of Gastroenterology, Hospital Afftiated to Jiangsu University, Jiangsu, Zhenjiang 212001 |
| Abstract: | Objective:To study the effects of STAT3 small interfering RNA(siRNA)on invasion of colon cancer cell line HCT116.Methods:After colon cancer HCT116 cell were transfected by STAT3 siRNA,we studied the expression of STAT3 mRNA using RT-PCR,anchorage-independent growth using clon formation in soft agar,and invasion using boyden chamber model,anoikis using and DNA fragmentation assay and terminal deoxynucleotidyhransferase-mediated dUTP nick-end labeling (TUNEL).Results:STAT 3 siRNA can inhibit anchorage-independent growth and invasion ability in a dose-dependent manner. STAT 3 can reduce the resistance to anoikis of HCT116 cells in a dose-and time-dependent manner.Conclusion:STAT 3 siRNA can in- hibit anchorage-independent growth and invasion ability of colon cancer cell through inducing anoikis. |
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