重组人促红细胞生成素对人脐静脉内皮细胞PCNA表达的影响

目的研究重组人促红细胞生成素（rHuEPO）对人脐静脉内皮细胞（HUVEC）增殖的影响。方法从脐静脉体外分离培养HUVEC，采用细胞培养及免疫组织化学方法检测不同剂量rHuEP0（10U／ml、20U／ml、40U／m1）对HUVEC增殖细胞核抗原（PCNA）表达的影响。结果对组照及10U／ml、20U／ml、40U／mlrHuEP0实验组内皮细胞PCNA阳性表达百分率分别为（31±3）％、（40±5）％、（68±6）％和（65±5）％，与对照组相比，rHuEPO作用后内皮细胞PCNA表达明显增强（P〈0．05），且随rHuEPO剂量提高作用增强，呈剂量效应关系。结论rHuEP0可促进体外培养内皮细胞增殖。

Effects of Recombinant Human Erythropoietin on The Expressipn of The Proliferating Cell Nuclear Antigen in Endothelial Cells from Human Umbilical Veins

Objective To study the effects of recombinant human erythropoietin on the the proliferation of endothelial cells from human umbilical veins Methods The endothelial cells were isolated from human umbilical vein and cultured with 10U/ml, 20U/ml and 40U/ml rHuEPO, respectively. Then the expression of proliferating cell nuclear antigen （PCNA） was detected using immunohistochemical methods. ResultsThe positive percentage of PCNA in HUVECs from control group, 10U/ml rHuEPO group , 20U/ml rHuEPO group and 40U/ml rHuEPO group were （31±3） %, （40±5） %, （68±6） % and （65±5） %, respectively. Compared with the control group, the proliferation of endothelial cell in rHuEPO group enhanced significantly（P〈0. 05）in a dose-dependent manner. Conclusion rHuEPO can promote the proliferation of endothelial cell cultured in vitro.