Rapid on-site detection of Acidovorax citrulli by cross-priming amplification |
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| Authors: | Zhang Jing Tian Qian Zhu Shui-fang Zhao Wen-jun Liu Feng-quan |
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| Affiliation: | College of Plant Protection, Nanjing Agricultural University, China. |
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| Abstract: | Cross-priming amplification (CPA) for Acidovorax citrulli detection was evaluated in this study. The sensitivity of CPA assay for pure bacterial culture was 3.7 × 10(3) CFU/ml. Bacteria on naturally infected watermelon seeds were detected using CPA assay, suggesting this method is suitable for A. citrulli on-site detection from watermelon seeds. |
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