双歧杆菌的完整肽聚糖对大鼠腹腔巨噬细胞ERK的影响

目的探索分叉双歧杆菌的完整肽聚糖(WPG)对大鼠腹腔巨噬细胞ERK1/2的调控作用。方法以WPG刺激大鼠腹腔巨噬细胞或以PKC抑制剂Chelerythrine预先孵育巨噬细胞,再用WPG刺激巨噬细胞,最后用Western blot检测巨噬细胞ERK1/2的表达水平。结果未受WPG刺激的正常大鼠腹腔巨噬细胞ERK1/2处于低活性状态,给予WPG刺激巨噬细胞,其磷酸化ERK1/2的蛋白表达水平明显高于对照组(P<0.01)。但经Chelerythrine预先孵育巨噬细胞后,其磷酸化ERK1/2的蛋白表达水平明显低于WPG刺激组(P<0.01)。结论分叉双歧杆菌的WPG可通过PKC激活巨噬细胞的ERK1/2。

Regulation of whole peptidoglycan of Bifidobacteria on ERK of rat peritoneal macrophages

Aim To explore the regulation of whole peptidoglycan (WPG)of Bifidobacteria bifidum on ERK of rat peritoneal macrophages.Methods Rat peritoneal macrophages were stimulated by WPG. Macrophages were pretreated with PKC inhibitor Chelerythrine and stimulated by WPG.The expression of ERK of the macrophages was detected by using Western blot.Results The ERK activity was low in normal rat peritoneal macrophages. After the macrophages were stimulated by WPG,its protein expression level of phosphorylation of ERK1/2 was obviously higher when compared with control group(P<0.01). After the macrophages were pretreated with Chelerythrine,its protein expression level of phosphorylation of ERK1/2 was markedly lower when compared with WPG stimulating group(P<0.01).Conclusion WPG of Bifidobacteria bifidum could activate ERK of macrophages through PKC.