自体游离骨膜骨髓自固化磷酸钙复合移植治疗低负重或非负重部位节段性骨缺损的实验研究

背景骨膜移植及骨移植是目前治疗节段性骨缺损的常用方法,疗效尚未令人满意.目的探讨自体游离骨膜骨髓自固化磷酸钙(FP-BM-CPC)复合移植修复节段性骨缺损的效果.设计自身对照的前瞻性研究.地点和对象实验由桂林医学院附属医院骨科和广西医科大学第一附属医院创伤骨科共同完成,研究对象为新西兰兔15只,雌雄不限,体质量1.5～2.5 kg,由广西医科大学动物实验室提供.方法制造实验兔双侧桡骨中段10mm的骨缺损模型.实验组一侧骨缺损用FP-BM-CPC复合移植修复;对侧作为对照组.主要观察指标术后1,2,4,6,8周行双侧桡骨X线平片及组织学检查.结果实验组兔桡骨骨缺损处在8周时均获得骨性修复,对照组均为骨不连.结论FP-BM-CPC复合移植具有良好的成骨作用,可望成为修复低负重或非负重部位节段性骨缺损的有效方法.

Study of united transplantation of free periosteum-bone marrow-calcium phosphate in the treatment of segmental bone defect at low-bearing or non-bearing parts

BACKGROUND: Periosteum transplantation and bone transplantation are common methods in the treatment of segmental bone defect at present with unsatisfied effectiveness.OBJECTIVE: To discuss effectiveness of united transplantation of free periosteum-bone marrow-calcium phosphate(FP-BM-CPC) in the restoration of segmented bone defect.DESIGN: A self-control prospective study was performed.SETTING and PARTICIPANTS: The study was completed in the Department of Orthopaedics, the Affiliated Hospital of Guiltn Medical College and Department of Orthopaedics and Traumatology, the First Affiliated Hospital of Guangxi Medical University. Subjects were 15 New Zealand rabbits in either genders and with a body weight between 1.5 to 2.5 kg provided by Laboratory of Experimental Animals, Guangxi Medical University.METHODS: A bone defect model at 10 mm of midpiece in both radiuses of trialal rabbit was established. Trial group: one side of bone defect was restored by the transplantation of FP-BM-CPC; and the other side was taken as control group.MAIN OUTCOME MEASURES: X-ray plain films and histological examinations for both radiuses after 1,2, 4, 6 and 8-week surgery.RESULTS: Bone defects in trial group were restored after 8-week injury but disunion in control group.CONCLUSION: United transplantation of FP-BM-CPC has good osteogenesis, which hopefully can be used as an effective method in the restoration of segmental bone defects in low-bearing or non-bearing parts.