胚胎期不同药物诱导大鼠隐睾模型的比较

Objective To establish the rat model of cryptorchidism induced by fetal drug exposure. Methods Pregnant SD rats were randomly assigned into 5 groups according the treatment.From the gestational day 12 to day 21, the rats of the DBP group were administered with dibutyl d-1) and diethylstilbestrol (DES), respectively. The normal pregnant rats were injected subcutanecontrols. The testes were removed on postnatal day 20 and day 80 (PD20 and PD80). The incidence of cryptorchidism was recorded. The testes' morphology and histology were also studied. Results The fetal DES exposure failed to induce cryptorchidism. Of the Flu rats on PD20 and PD80, testis volumes were ( 123. 27 ± 7. 09)mm3 , and (0. 96 ± 0. 26)cm3 , respectively. The organ coefficients were (2. 27 ±0. 09)‰, and ( 1.94 ± 0. 62)‰, respectively. Mean seminiferous tubule diameters (MSTD) were (91.50 ± 6. 29)μm and ( 161.26 ± 33. 80)μm, respectively. Mean seminiferous tubule areas (MSTA)were (6. 62 ± 0. 90 ) × 103 μm2 and (2. 00 ± 1.00) × 104μm2 and ( 1.19 ± 0. 24) × 1 04μm2 , respectively. Of the DBP rats on PD20 and PD80, testis volumes were (117. 39 ± 8. 00)mm3, and (0. 79 ± 0. 28)cm3 , respectively. The organ coefficients were (2. 26 ± 0. 09)‰, and (2. 20 ± 0. 87)‰, respectively.Mean seminiferous tubule diameters (MSTD) were (94. 74 ± 6. 75)μm, and (122. 20 ± 12. 55)μm, respectively. Mean seminiferous tubule areas (MSTA) were (7. 14 ± 1.03) × 103 μm2, and ( 1.19 ±0. 24) × 104 μm2, respectively. The testis volumes, organ coefficient, MSTD and MSTA of the Flu and DBP rats on PD20 and PD80 were significant smaller than those of the controls (P＜0. 01 ). The presences of gonocytes were noted in the center of cryptorchidism seminiferous tubules on PD20 and PD80, which indicated the fetal Flu exposure delayed the lumen formation of the seminiferous tubules.No gonocytes was found in the cryptorchidism seminiferous tubules of the DBP rats. Conclusions The crytorchidism can be induced by fetal Flu exposure characterized by gonocytes malmigration and delayed seminiferous tubules formation. It is a good model to explore the gonocytes development in cryptorchism.

Expermetnal cryptorchidism induced by fetal drug exposure in rats