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人类脂肪来源成体干细胞体外定向成软骨的诱导实验
引用本文:雷紫雄,廖威明,盛璞义,付明,何爱珊,杨子波.人类脂肪来源成体干细胞体外定向成软骨的诱导实验[J].现代临床医学生物工程学杂志,2010,17(5):4-8.
作者姓名:雷紫雄  廖威明  盛璞义  付明  何爱珊  杨子波
作者单位:中山大学附属第一医院关节外科,广州,510080;
基金项目:广东省自然科学基金项目
摘    要:Objective To investigate the feasibility of inducing human adipose derived adult stem cells (hADASc) into chondrocytes by gene transfection.Methods hADASc were cultured in vitro, and transfected with gene PGL3-TGF-pl, whose Luceferase activity (RUL value) was measured.PT-PCR was used to monitor the expression of tranfected hADASc.Anti collagen II immunohistochemical staining was performed in the experimental group (transfected hADASc) , active control group (untransfected hADASc cultured in chondrocytes induction medium) and negative control group (untransfected hADASc cultured in conventional medium).The immuno-staining image was analyzed with an automated imaging analysis system and computed for PU values.Results Nucleated tissue cells with features of stem cells were isolated from in vitro culture of human subcutaneous adipose tissue.The RUL value of the experimental group (9 212.583±315.240) was higher than that of the control group(317.000?0.710, P<0.01).RT-PCR revealed that the transfected hADASc could express the TGF-β1.Anti collagen Ⅱ immunohistochemical staining was positive in experimental group and active control group, with PU values (13.864±2.416 and 13.637±2.548,respectively) statistically different from that of the negative control group (6.013 ±0.827, P<0.05).Conclusions Nucleated tissue cells with features of stem cells can be isolated from culture of human subcutaneous adipose tissue in vitro.The PGL3-TGF-β1-transfected hADASc may express TGF-β1.The endogenous TGF-β1-induced hADASc produces collagen Ⅱ , with similar actions of exogenous TGF-β1.

关 键 词:成体干细胞    转染    软骨发生    基因    转化生长因子β1    Transforming  growth  factor  β1    

Experimental induction of human adipose derived adult stem cells (hADASc) into chondrocytes in vitro
LEI Zi-xiong,LIAO Wei-ming,SHENG Pu-yi,FU Ming,HE Ai-shan,YANG Zi-bo.Experimental induction of human adipose derived adult stem cells (hADASc) into chondrocytes in vitro[J].Journal of Modern Clinical Medical Bioengineering,2010,17(5):4-8.
Authors:LEI Zi-xiong  LIAO Wei-ming  SHENG Pu-yi  FU Ming  HE Ai-shan  YANG Zi-bo
Abstract:
Keywords:Adult stem cellsTransfectionChondrogenesisGenes
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