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| 高效液相色谱法测定灭澳灵胶囊中异嗪皮啶的含量 | |
| 引用本文： | 龚咏梅,王羚郦,马小泉,刘小宝,徐平声.高效液相色谱法测定灭澳灵胶囊中异嗪皮啶的含量[J].中南药学,2009,7(12):911-913. |
| 作者姓名： | 龚咏梅 王羚郦 马小泉 刘小宝 徐平声 |
| 作者单位： | 1. 新余市人民医院,江西,新余338025 2. 广州中医药大学中药学院,广州,510006 3. 江西青春康源制药有限公司,江西,新余338000 4. 新余市食品药品检验所,江西,新余338000 5. 中南大学湘雅医院,长沙,410008 |
| 摘 要： | 目的建立灭澳灵胶囊中异嗪皮啶的含量测定方法。方法采用高效液相色谱法进行测定。测定条件为Kro-masil C18（250 mm×4.6 mm,5μm）柱;流动相：乙腈-0.1%磷酸（15∶85）;流速：1.0 mL.min-1,柱温：30℃,检测波长为343 nm。结果异嗪皮啶在0.098 4~0.984μg与峰面积线性关系良好,得回归方程：Y=3.69×106X-2.855×103,r=0.999 8,平均回收率为98.82%,RSD=0.85%（n=9）。结论该方法简便、快速、准确,可用于灭澳灵胶囊中异嗪皮啶的定量分析。 |
| 关 键 词： | 高效液相色谱法 灭澳灵胶囊 异嗪皮啶 |
Determination of isofraxidin in Mieaoling capsules by HPLC |
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| GONG Yong-mei,WANG Ling-li,MA Xiao-quan,LIU Xiao-bao,XU Ping-sheng.Determination of isofraxidin in Mieaoling capsules by HPLC[J].Central South Pharmacy,2009,7(12):911-913. | |
| Authors: | GONG Yong-mei WANG Ling-li MA Xiao-quan LIU Xiao-bao XU Ping-sheng |
| Institution: | 1.Xinyu People＇s Hospital,Xinyu Jiangxi 338025;2.College of Chinese Materia Medica,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006;3.Jiangxi Qingchunkang Pharmaceutical Limited Company,Xinyu Jiangxi 338000;4.Xinyu Institute of Food and Drug Control,Xinyu Jiangxi 338000;5.Xiangya Hospital,Central South University,Changsha 410008) |
| Abstract: | Objective To establish a method to determine isofraxidin in Mieaoling capsules.Methods HPLC method was used.The chromatographic conditions were as follows：Kromasil C18 column（4.6 mm×250 mm,5 μm）;the mobile phase consisted of acetonitrile-1% H3PO4（15∶85）;the flow rate was 1.0 mL·min-1;column temperature was 30 ℃;and detective wavelength was 343 nm.Results The calibration curve was linear at 0.098 4~0.984 μg（r=0.999 8）.The average recovery rate was 98.82%（n=9）,and RSD was 0.85%.Conclusion The method is rapid,accurate and reliable,and can be used to determine isofraxidin in Mieaoling capsules. |
| Keywords: | HPLC Mieaoling capsule isofraxidin |
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