次级淋巴组织趋化因子及其受体CCR7在胰腺癌中的表达及临床意义

目的 研究胰腺癌中次级淋巴组织趋化因子(SLC)及其受体CCR7的表达情况,探讨其与胰腺癌临床病理关系.方法 应用免疫组化、RT-PCR和实时荧光定量PCR技术检测30例胰腺癌组织、癌旁组织、正常胰腺组织和胰周淋巴结组织中SLC和CCR7的表达情况.结果 SLC蛋白在胰腺癌组织中呈低表达状态、在癌旁组织和胰周淋巴结均呈中等表达状态、在正常胰腺组织中高表达,阳性率分别为16.7%(5/30)、43.3%(13/30)、46.6%(14/30)和76.7%(23/30).RT-PCR和实时荧光定量PCR也证实SIC mRNA表达与SIC蛋白相同.CCR7蛋白在胰腺癌组织、癌旁组织和胰周淋巴结均呈高表达状态、在正常胰腺组织中呈低表达状态,阳性率分别为76.7%(23/30)、66.7%(23/30)、70.0%(/30)和30.0%;同时还发现CCR7蛋白在胰腺静脉平滑肌和淋巴结外脂肪组织也有阳性表达情况.RT-PCR和实时荧光定量PCR结果 也证实CCR7 mRNA表达与CCR7蛋白相同.结论 SLC在胰腺癌进展和淋巴结转移过程中起双重作用,既发挥抗肿瘤作用,又通过趋化CCR7表达阳性的肿瘤细胞促进胰腺癌的淋巴结转移.CCR7与胰腺癌淋巴结转移和TNM分期密切相关,并可能参与胰腺癌的淋巴管生成和淋巴结转移的调控.

Expressions of secondary lymphoid-tissue chemokine and CCR7 in pancreatic cancer and their clinical significance

Objective To study the expressions of secondary lymphoid-tissue chemokine(SLC)and CCR7 and their correlation with clinical pathology in pancreatic adenocarcinoma(PAC).Methods The tissue samples,including PAC tissues,the cancerous peripheral tissues,and peripheral lympho nodes were obtained from 30 patients with PAC,while normal pancreatic tissues(30 samples)were also prepared.The expressions of SLC and CCR7 proteins in these tissues were assayed by immunohistochemical staining.The expressions of SLC and CCR...