| 利福平对异烟肼在小鼠原代肝细胞内代谢的影响 |
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| 引用本文： | 蔡大伟,侯艳宁,鲁传冬,李亚楠,薛改.利福平对异烟肼在小鼠原代肝细胞内代谢的影响[J].解放军药学学报,2009,25(5):414-416. |
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| 作者姓名： | 蔡大伟 侯艳宁 鲁传冬 李亚楠 薛改 |
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| 作者单位： | 1. 解放军第251医院,河北,张家口,075000
2. 白求恩国际和平医院,河北,石家庄,050082 |
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| 摘 要： | 目的探讨异烟肼（INH）与利福平（RFP）合用致肝毒性增加的部分作用机理。方法采用在体心脏灌流法获取小鼠原代肝细胞,将其常规培养5d后,分别用含INH（87.5μmol.L^-1）和INH＋RFP（87.5μmol.L^-1＋48.6μmol.L^-1）培养液培养48h,液相色谱-电喷雾质谱联用法测定2组培养液中INH及其代谢产物乙酰肼（Acetylhydrazine,AHZ）和肼（Hydrazine,HZ）的浓度。结果与INH组比较,INH＋RFP组AHZ和HZ浓度增加,有显著性差异（P〈0.01）。结论RFP和INH合用后使INH肝毒性代谢物浓度增加,可能是其肝毒性增加的原因之一。
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| 关 键 词： | 异烟肼 利福平 代谢 |
The Effect of Rifampicin on the Metabolism of Isoniazid |
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| CAI Da-Wei,HOU Yan-Ning,LU Chuan-Dong,LI Ya-Nan,XUE Gai.The Effect of Rifampicin on the Metabolism of Isoniazid[J].Pharmaceutical Journal of Chinese People's Liberation Army,2009,25(5):414-416. |
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| Authors: | CAI Da-Wei HOU Yan-Ning LU Chuan-Dong LI Ya-Nan XUE Gai |
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| Institution: | 1.Hospital 251 of PLA,Zhangjiakou 075000,Hebei China;2.Bethune International Peace Hospital, Shijiazhuang 050082, Hebei China) |
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| Abstract: | Aim To investigate the mechanism of isoniazid （INH）and rifampicin（RFP）. Methods Mouse hepatocytes were obtained through cardio-perfusion in mice and isolated by two-step in situ collagenase perfusion methods, after five days of culture of mouse hepatocytes, the culture hquid was replaced by INH（87.5μmol.L^-1 ） , INH ＋ RFP（87.5μmol.L^-1 ＋48.6μmol.L^-1 ）. the concentration of AHZ and HZ was determined by LC/ESI-MS in 48h. Results The concentration of AHZ and HZ increased more in the INH ＋ RFP group than in the INH group（ P 〈 0.01 ）. Conclusion RFP can increase the concentration of acetylhydrazine （AHZ） and （ hydrazine ） HZ, which may increase the hepatotoxicity of INH. |
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| Keywords: | isoniazid rifampicin metabolism |
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