| 入胎脑未知基因EST的发现 |
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| 引用本文： | 关敏,孙蕾,常雅萍,于永利. 入胎脑未知基因EST的发现[J]. 吉林大学学报(医学版), 2001, 27(6): 589-591 |
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| 作者姓名： | 关敏 孙蕾 常雅萍 于永利 |
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| 作者单位： | 吉林大学基础医学院免疫学教研室,吉林,长春,130021 |
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| 摘 要： | 目的:在人胎儿脑组织cDNA中钓取并克隆新基因.方法:以RT-PCR方法用人FGF-19特异性引物从人胎脑cDNA中扩增到一大小约660bp的片段,并连入TA克隆载体测序,得到一个表达序列标签(EST),输入GeneBank、Swissprot、dbEST等数据库中进行同源比较分析.结果:分析表明它是人胎儿脑组织未知基因EST,是一个663个碱基可编码220个氨基酸的开放读码框架.结论:在人胎儿脑组织cDNA中钓取并克隆一个人胎脑新基因EST.
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| 关 键 词： | 表达序列标签 cDNA RT-PCR |
| 文章编号： | 0253-3707(2001)06-0589-03 |
| 修稿时间： | 2000-10-21 |
Discovery of a novel EST of human fetal brain |
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| GUAN Min,SUN Lei,CHANG Ya ping,YU Yong li. Discovery of a novel EST of human fetal brain[J]. Journal of Jilin University: Med Ed, 2001, 27(6): 589-591 |
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| Authors: | GUAN Min SUN Lei CHANG Ya ping YU Yong li |
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| Abstract: | Objective:To obtain and clone the new gene of human fetal brain. Methods: A cDNA fragment about 660 bp was isolated from human fetal brain tissue by RT PCR with human FgF 19 primers. Then it was cloned into pGEM T Easy plasmid to be sequenced. Results: Sequencing analysis proved that it was a novel Expressed Sequence Tag(EST) derived form human fetal brain and contained an open reading frame(ORF) with the size of 663 bp coding for 220aa.Conclusion: A human novel EST was obtained. |
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| Keywords: | EST cDNA RT PCR |
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