复合因素致慢性疲劳大鼠运动能力及血生化的变化

摘要]目的 观察不同强度睡眠剥夺复合力竭游泳作用因素导致的慢性疲劳大鼠运动能力及血液生化指标的变化。方法 32只雄性SPF级SD大鼠分为正常对照组、20h、16h、12h模型组，将模型组大鼠放入水深1.5cm的盒子分别20h、16h、12h进行睡眠剥夺。将模型组大鼠负重其体重5%放入水深50cm的玻璃缸中游泳至力竭，记录力竭游泳时间。第14d力竭游泳后12h处死大鼠，进行血液生化的检测。结果 20h模型组负重力竭游泳时间随时间显著降低(P<0.01)；16h、12h模型组力竭游泳时间降低不明显。与正常对照相比较，20h模型组AST、UA、LDH明显升高（Р<0.01），ALB、Scr、GLU、Tch、TG明显降低（Р<0.05），ALT、TP、TBIL、BUN、CRP、CK差异无统计学意义（Р>0.05）；16h模型组、12h模型组标差异均无统计学意义（Р>0.05）。结论 睡眠剥夺20h复合负重其体重的5％力竭游泳使大鼠运动能力下降，并引起AST、UA、LDH 升高，Scr、GLU、Tch、ALB、TG降低。

Changes of Swimming Ability and Blood Biochemical Index among the Chronic Fatigue Rat by Compound Factors

Objective To observe the changes of swimming ability and blood biochemical index among chronic fatigue rat by compound factors of different degrees of sleep deprivation combined with exhaustive swimming exercising.Methods 32 male SD rats of SPF grade were selected and randomly divided into 4 groups: Normal control group (n=8), 20-hour sleep deprivation group (n=8), 16-hour sleep deprivation group(n=8) and 12-hour sleep deprivation group (n=8). Rats were differently kept in a cage filled with water to a height of 1.5cm for 20 hours, 16 hours and 12 hours, after that, rats in the three groups were required to conduct exhaustive swimming in glass pool with load 5% of weight of the rat for 14 days. We selected a weight-loaded forced swimming test for evaluation of the extent of fatigue. Exhaustive swimming standard was 10 seconds after rats sank into water without outcrop. Rats in the normal control group did not deprive sleep and swimming and were fed routinely. Rats in each group were executed after exercise to determine the blood biochemical index. We observe the exhaustive swimming time and biochemical index.Results The exhaustive swimming time of rats in 20-hour sleep deprivation group was significantly shortened (P<0.01); Compared with normal control group, the level of AST, UA, LDH significantly increased and the level of Alb, Scr, GLU, Tch and TG significantly decreased and the other level did not change among the rats in 20-hour sleep deprivation group. The 16-hour and 12-hour sleep deprivation group did not have significant change.Conclusion The 20-hour sleep deprivation combined with weight-loaded forced exhaustive swimming can decrease the swimming capability and increase the level of AST, UA and LDH and decrease the level of Scr, GLU, Tch, Alb and TG.