长链非编码RNA SFTA1P在非小细胞肺癌组织中的表达和细胞功能研究

  目的  研究长链非编码RNA SFTA1P在非小细胞肺癌(non-small cell lung cancer, NSCLC)中的表达情况, 以及对NSCLC细胞系生物学功能的影响。  方法  运用定量即时聚合酶链锁反应(quantitative real time polymerase chain reaction, qRT-PCR)检测SFTA1P在18对非小细胞肺癌组织及癌旁组织中的表达情况; 用qRT-PCR检测5株NSCLC细胞系(A549、SPCA1、H1975、H460和H1299)和1株肺正常上皮细胞系(human bronchial epithelial, HBE)中SFTA1P的表达情况; 构建SFTA1P的过表达载体, 通过转染构建过表达细胞模型; 运用CCK-8、Transwell等实验方法检测SFTA1P过表达对NSCLC细胞增殖、侵袭、迁移的影响。  结果  SFTA1P在非小细胞肺癌组织中的表达低于癌旁组织(t=2.158, P=0.043);与HBE细胞相比较, SFTA1P在A549和H460细胞系中相对低表达(t=5.769, P=0.004;t=5.772, P=0.004), 在H1299和H1975细胞系中相对高表达(t=22.248, P < 0.001;t=11.814, P < 0.001);SFTA1P过表达质粒转染A549和H460细胞系后, 与空质粒的对照组比较, SFTA1P的表达均升高, 且有统计学差异(均有P < 0.05);过表达SFTA1P可以抑制非小细胞肺癌细胞H460、A549的增殖、侵袭、迁移, 差异有统计学意义(均有P < 0.05)。  结论  SFTA1P基因能够抑制NSCLC细胞的增殖、迁移、侵袭等生物学功能, SFTA1P在肿瘤发生和发展中可能起着抑癌基因的作用。

Expression and cellular function of long non-coding RNA SFTA1P in non-small cell lung cancer

Objective To investigate the expression of long non-coding RNA SFTA1 P in non-small cell lung cancer(NSCLC)and its biological function in NSCLC cell lines.Methods Quantitative real time polymerase chain reaction(qRT-PCR)was used to detect the expression of SFTA1 P in 18 pairs of NSCLC tissues and adjacent normal tissues.The expression of SFTA1 P was detected by qRT-PCR in five different NSCLC cell lines(A549,SPCA1,H460,H1975 and H1299)and one normal lung epithelial cell line(HBE).The overexpression vector of SFTA1 P was designed and constructed.The overexpressed cell line was constructed by transfection,the effects of overexpression of SFTA1 P on proliferation,invasion and migration of NSCLC cells were detected by CCK-8 assay and transwell assay.Results The expression of SFTA1 P in NSCLC tissues was lower than that of adjacent normal tissues(t=2.158,P=0.043).SFTA1 P expression was detected in 5 strains of NSCLC cell lines and normal lung epithelial cell line.The expression of SFTA1 P was the lowest in A549 and H460 cell lines(t=5.769,P=0.004;t=5.772,P=0.004),and the highest in H1299 and H1975 cell lines(t=22.248,P<0.001;t=11.814,P<0.001).SFTA1 P overexpression cell models were successfully constructed using A549 and H460 cell lines(all P<0.05).The overexpression of SFTA1 P could inhibit proliferation,invasion and migration of H460 and A549 cells(all P<0.05).Conclusions SFTA1 P can affect the biological functions of NSCLC cells by inhibiting the proliferation,migration and invasion.SFTA1 P may play a role as a tumor suppressor gene in tumorigenesis and development.