组蛋白去乙酰化酶基因多态性和环境因素对2型糖尿病的影响

目的探讨组蛋白去乙酰化酶(histone deacetylase,HDACs)的3个单核苷酸多态性位点与2型糖尿病(type 2 diabetes mellitus,T2DM)的关联,以及各位点与环境间交互作用对T2DM易感性的影响,为T2DM预防和治疗提供依据。方法2017年在广州市花都区17个行政村和2个社区居委中随机抽取10个村做基线调查后选取T2DM者为病例组,以同性别、年龄相差不超过5岁和来源于同一行政村为条件,1∶1个体匹配方法匹配对照组。采用条件Logistic回归分析模型分析基因和环境及其交互作用对T2DM的影响。结果共调查499名病例,平均年龄(61.53±13.08)岁,男性占37.88%;499名对照的平均年龄(61.48±13.09)岁,男性占37.88%。甘油三酯(triglyceride,TG)异常(OR=2.410,95%CI:1.755~3.310,P<0.001)和总胆固醇(total cholesterol,TC)异常(OR=1.436,95%CI:1.046~1.972,P=0.025)是T2DM的危险因素。rs72792338 TT+TC基因型携带(OR=0.526,95%CI:0.349~0.793,P=0.002)是T2DM的保护因素。结论甘油三酯和总胆固醇异常是T2DM的危险因素,rs72792338 TT和TC基因型携带是T2DM的保护因素,未发现HDACs基因与环境对T2DM的影响存在交互作用。

Study on the effect of histone deacetylase gene polymorphism and environmental factors on type 2 diabetes mellitus

  Objective  To examine whether polymorphisms of histone deacetylase(HDACs) and environment factors can be implicated in type 2 diabetes mellitus(T2 DM), and to provide evidence for the prevention and treatment of T2 DM.  Methods  In 2017, T2 DM patients and controls were selected from 17 villages in Huadu District, Guangzhou. According the Diagnostic criteria for T2 DM, the case group of T2 DM was matched with control group from the population diagnosed as normal by gender, age no more than 5 years old, and from the same natural village. Conditional logistic regression model was used to analyze the effect of gene and environment and their interaction on T2 DM.  Results  The average age of 499 cases group were(61.53±13.08) years old, and the average age of 499 controls group were(61.48±13.09) years old. There were no statistic difference between two groups. Furthermore, the two groups were gender-balanced too. In conditional logistic regression model, we found that glycerin trilaurate(TG) abnormalities(OR=2.410, 95% CI: 1.755-3.310, P < 0.001) and cholesterol total(TC) abnormalities(OR=1.436, 95% CI: 1.046-1.972, P=0.025) were risk factors for T2 DM. The subjects carries rs72792338 TC+TT genotype(OR=0.526, 95% CI: 0.349-0.793, P=0.002) had lower the risk to develop T2 DM.  Conclusions  Abnormal TG and TC are risk factors for T2 DM. Rs72792338 TT and TC genotype carryings decrease the risk of T2 DM.