禽流感H5N1病毒感染ICR小鼠的病理变化

 【目的】 通过鼻腔将H5N1禽流感病毒接种ICR小鼠,观察其主要器官组织的病理变化?【方法】 麻醉ICR小鼠后将100 μL H5N1禽流感病毒原液用移液枪滴入接毒组小鼠鼻腔,接毒后14 d内每隔24 h取材一次,采用福尔马林固定,石蜡包埋?切片,HE染色?【结果】 禽流感H5N1病毒感染ICR小鼠后表现出明显的病理改变?ICR小鼠的肺部病变最严重,表现为间质性肺炎,肺间质充血?水肿和淋巴细胞浸润,血管周围淋巴细胞浸润,毛细血管扩张,上皮细胞变性?坏死?脱落,并有充血和单核细胞浸润,肺泡壁明显增宽,血管充血,有的肺泡溶合,呈气肿状?肝?肾?脑等其它脏器也出现病变?【结论】 禽流感ICR小鼠模型能复制出人类禽流感疾病的许多病理特征,禽流感H5N1病毒感染ICR小鼠的肺病理类似于人禽流感严重病例的肺病理?

Pathological Change for ICR Mice Infected with Avian Influenza H5N1 Viruses

[Objective] The pathologic changes were observed after ICR(Institute of cancer Research) mice were infected with avian influenza A (H5N1) viruses through the nasal cavity. [Methods] Anesthetized ICR mice were inoculated intranasally with avian influenza virus isolates at a biosafety laboratory. Throughout the 14-day experiment tissue samples from lungs,livers,kidneys etc. were processed for histopathology. [Result] The ICR mice exhibited severe pathologic changes, multiple systemic organs hurt, including the brain. The lung tissues showed interstitial pneumonia, dropsy, configuration breakage, broadened alveolar septa, epithelial desquamation and putrescence, capillary vessel dilatation and congestion, and interstitial lymphocyte infiltration. [Conclusion] The pathology of the lungs of infected ICR mice was similar to that of severe cases of avian influenza in humans.