2,3,6- Tribromo- 4,5- dihydroxybenzyl Methyl Ether Induces Growth inhibition and apoptosis in MCF-7 human breast cancer cells |
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Authors: | Ji-Hyeon Lee Sang Eun Park Mohammad Akbar Hossain Min Young Kim Mi-Na Kim Hae Young Chung Jae Sue Choi Young Hyun Yoo Nam Deuk Kim |
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Institution: | Division of Pharmacy (BK21 Program), College of Pharmacy, Research Institute for Drug Development, Pusan National University, Busan 609-735, Korea. |
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Abstract: | In this study, we investigated the effects of 2,3,6-tribromo-4,5-dihydroxybenzyl methyl ether (TDB), isolated from Symphyocladia latiuscula (marine red algae), on the proliferation of MCF-7 human breast cancer cells. TDB treatment for 48 h inhibited cancer cell growth and induced DNA fragmentation. Furthermore, morphological characterizations such as apoptotic bodies and membrane blebs were shown by electronic microscopy. TDB-induced apoptosis in the MCF-7 cells was closely linked with the down-regulation of Bcl-2 protein expression and the cleavage of caspase-3 substrates, with poly(ADP-ribose) polymerase cleavage occurring by TDB treatment. TDB treatment also caused a marked increase in the level of p21WAF1/CIP1 protein in a p53-dependent manner. In addition, the upregulation of p21WAF1/CIP1 in the MCF-7 cells was related to a decrease in c-Myc protein in a dose-dependent manner. Based on our data, TDB is a good candidate for further evaluation as an effective chemotherapeutic agent, acting through the induction of apoptosis. |
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Keywords: | 2 3 6-Tribromo-4 5-dihydroxybenzyl methyl ether Cytotoxicity Apoptosis Human breast cancer cells |
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