| 强力霉素抑制MMP-9对大鼠局灶性缺血再灌注脑损伤的保护作用 |
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| 引用本文： | 焦海霞,王萍,马腾,薛一雪.强力霉素抑制MMP-9对大鼠局灶性缺血再灌注脑损伤的保护作用[J].第三军医大学学报,2010,32(24). |
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| 作者姓名： | 焦海霞 王萍 马腾 薛一雪 |
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| 作者单位： | 1. 110001,沈阳,中国医科大学基础医学院神经生物学教研室;350004,福州,福建医科大学基础医学院生理学与病理生理学系
2. 中国医科大学基础医学院神经生物学教研室,沈阳,110001 |
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| 基金项目： | 国家自然科学基金(3087265630973079); 教育部高等学校博士学科点专项科研基金(20092104110015); 沈阳市科学技术计划项目(1091175-1-01,1081266-9-00)~~ |
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| 摘 要： | 目的观察强力霉素对脑缺血再灌注MMP-9活性的影响,探讨其对神经元的保护作用。方法利用大脑中动脉线栓法(middle cerebral artery occlusion,MCAO)制备局灶性脑缺血再灌注模型,SD大鼠分为假手术组、生理盐水组、强力霉素组,每组18只。激光多普勒血流仪分别检测大鼠手术前、MCAO时、再灌注24 h大脑中动脉脑血流;再灌注24 h时应用TTC染色法观察大鼠脑梗死体积;明胶酶谱法检测缺血区脑组织MMP-9活性变化;透射电镜观察缺血区脑组织神经元超微结构。结果生理盐水组与强力霉素组大鼠在再灌注24 h时脑血流分别为基础值的(57.98±4.20)%、(59.23±3.30)%]与MCAO时分别为基础值的(22.94±3.16)%、(21.63±3.46)%]差异显著(P<0.01),但在同一时间点两组间无显著差异(P>0.05);强力霉素组大鼠脑梗死体积(9.77±2.68)%]与生理盐水组(33.44±4.50)%]相比显著减少(P<0.01);生理盐水组大鼠MMP-9活性(5.95±0.73)与假手术组(1.21±0.15)相比显著升高(P<0.01),应用强力霉素后MMP...
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| 关 键 词： | 强力霉素 脑缺血 再灌注 基质金属蛋白酶 脑梗死 |
Doxycycline-mediated inhibition of MMP-9 protects focal cerebral ischemia-reperfusion injury in rats |
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| Jiao Haixia,Wang Ping,Ma Teng,Xue Yixue.Doxycycline-mediated inhibition of MMP-9 protects focal cerebral ischemia-reperfusion injury in rats[J].Acta Academiae Medicinae Militaris Tertiae,2010,32(24). |
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| Authors: | Jiao Haixia Wang Ping Ma Teng Xue Yixue |
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| Institution: | Jiao Haixia1,2,Wang Ping1,Ma Teng1,Xue Yixue1(1Department of Neurobiology,College of Basic Medicine,China Medical University,Shenyang,Liaoning Province,110001,2Department of Physiology and Pathophysiology,Fujian Medical University,Fuzhou,Fujian Province,350004,China) |
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| Abstract: | Objective To observe the activity alteration of MMP-9,and investigate the protective effect of doxycycline on brain tissue after cerebral ischemia-reperfusion injury in rats.Methods Cerebral ischemia-reperfusion model was induced by middle cerebral artery occlusion(MCAO).SD rats were divided into sham-operated group,saline group and doxycycline group,18 rats in each group.The laser-doppler probe was fixed before and after operation(MCAO),and at reperfusion 24 h,to measure local cerebral blood flow(CBF) in a... |
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| Keywords: | doxycycline brain ischemia reperfusion injury matrix metalloproteinases brain infarction |
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