脑肿瘤中P53基因突变与突变型P53蛋白表达的研究

采用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）法对４１例脑肿瘤进行Ｐ５３基因突变的检测，并与抗突变型Ｐ５３蛋白单抗的免疫组化染色结果相对照，结果显示脑肿瘤中Ｐ５３基因突变率为３４．１％（１４／４１），胶质瘤为２８％（１０／３６）。Ｐ５３基因突变与突变型Ｐ５３蛋白表达具有高度一致性，且二者均与脑肿瘤的分化程度有关。Ｐ５３基因突变及蛋白的表达不仅出现在高恶度胶质瘤及转移癌，而且可出现在低恶度胶质瘤中，表明Ｐ５３基因的突变在脑瘤的发生、发展过程中起重要作用。

P53 Gene mutation and P53 protein expression in brain tumors

P53 gene mutation in 41 brain tumors were analyzed by single-strand conformation polymorphism(SSCP),and the results were compared with P53 protein expression detected by immunohistochemistry.P53 gene mutations were found in 14 out of 41 brain tumors(34.1%),in 10 of 36 gliomas(28%).There was a highly significant association between the presence of P53 mutations and P53 nuclear reactivity(P<0.001).P53 alteration(mutation and expression)were related to grading of malignancy of brain tumors.P53 gene mutation was found not only in high-malignant glioma,but also in low-malignant glioma.This indicates that P53 gene plays an important role in the development transformation of brain tumors,and malignant tumors.