| 44例人脑胶质瘤P53基因突变的研究 |
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| 引用本文： | 刘爱学 浦佩玉. 44例人脑胶质瘤P53基因突变的研究[J]. 中华神经外科杂志, 1996, 12(6): 347-350 |
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| 作者姓名： | 刘爱学 浦佩玉 |
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| 作者单位： | 天津医科大学总院脑外科，华北煤炭医学院附属医院脑外科 |
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| 摘 要： | 用ＰＣＲ－ＳＳＣＰ及ＤＮＡ直接测序研究了４４例人脑胶质瘤。发现１３例有Ｐ５３基因突变。３３例星形细胞瘤１２例有Ｐ５３基因突变。１１例非星形细胞瘤１例有Ｐ５３基因突变。ＤＮＡ直接测序证实所有突变均在Ｐ５３基因第５～８外显子，发现了一些新的突变位点，并发现在３例胶质母细胞瘤中存在Ｐ５３基因的多个位点的突变。该结果提示在大多数非星形细胞胶质瘤并不涉及Ｐ５３基因突变，Ｐ５３基因突变对星形细胞瘤的诊断有重要价值。特别是多位点的Ｐ５３基因突变可做为星形细胞瘤进展到胶质母细胞瘤的基因标志。
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| 关 键 词： | P53基因 突变 胶质瘤 |
StudyontheP53genemutationsin44humangliomas |
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| LiuAixue,PuPeiyu,ChengAiguo,et al.. StudyontheP53genemutationsin44humangliomas[J]. Chinese Journal of Neurosurgery, 1996, 12(6): 347-350 |
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| Authors: | LiuAixue PuPeiyu ChengAiguo et al. |
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| Affiliation: | LiuAixue,PuPeiyu,ChengAiguo,etal.De-partmentofNeurosurgery,TianjinMedicaUniversityGeneralHospitalTianjin300052 |
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| Abstract: | ByusingPCR-SSCPanalysisanddirectDNAsequencingofPCRproducts.P53genemutationswerestudiedin44casesofhumangliomas.13(29.50%)ofwhichwerefoundtohavetheP53genemutation.12mutationswerefoundin33astrocyticgliomas,onemutationwasfoundin11nonastrocyticgliomas.DNAsequencingindicatedthataloftheP53genemutationoccuredinits5th-8thexons,butsomenewspotsofP53genemutationsweredetectedinthepresentstudy.Furthermore,itwasfoundthatinthreeoutof13casesmutationsexamed,morethanonesiteofmutationexistedeveninasinglespeciumandalthesethreegliomaswereidentifiedhistopathologicalyasglioblastomas.ThisresultrevealedthatP53genemutationshaveanimportantdiagnosticvalueinastrocyticgliomas,butnotinvolvedinmostofnonastrocyticgliomas,andmultiplemutationofP53genemaybethemarkerofadevelopedhumanastrocytomaprogressedtoglioblastoma,anincurableendstage. |
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| Keywords: | P53geneMutationglioma |
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