内脏脂肪素和二甲双胍对多囊卵巢综合征大鼠胰岛素抵抗和生殖内分泌的治疗作用

目的比较内脏脂肪素（visfatin）和二甲双胍对多囊卵巢综合征（PCOS）大鼠胰岛素抵抗及生殖内分泌紊乱的治疗作用，为
visfatin进一步应用于PCOS治疗的临床研究提供理论依据。方法40只SD大鼠随机平均分为A、B、C、D组4组，A、B、C三组
采用DHEA造模法建模，通过连续阴道液涂片及血清学检测判断建模成功与否，A、B组为实验组，C组为PCOS建模对照组，D
组为空白对照组。建模成功后，A组大鼠予visfatin 10 μg/只单次给药，B组大鼠予二甲双胍14 mg/100 g体质量连续灌胃15 d。
干预前后采血测定T、LH、FSH、FINS 及OGTT各时段血糖，并计算HOMA-IR指数及LH/FSH 比值，最后剖取卵巢行病理切
片。结果（1）干预前后对比，A、B组大鼠血FINS、FPG、T、HOMA-IR及OGTT各时段血糖均显著降低（P<0.05）；（2）干预后，A
组和B组中分别有8只（80%）及7只（77.8%）大鼠恢复规律的发情周期，均可见正常卵泡的发育；（3）visfatin与二甲双胍在改善
PCOS大鼠胰岛素抵抗及高雄激素血症方面的疗效相同。结论visfatin和二甲双胍均能改善PCOS大鼠的胰岛素抵抗及高雄
激素血症，并能促进其规律动情周期的建立及正常卵泡的发育，且两者疗效相同。

Effects of visfatin and metformin on insulin resistance and reproductive endocrine in rats with polycystic ovary syndrome

Objective To investigate the therapeutical effects of visfatin and metformin on insulin resistance and reproductive
endocrine disorder in rats with polycystic ovary syndrome (PCOS). Methods Forty female Wistar rats were divided into 4
equal groups, and in groups A, B and C, the rats were injected subcutaneously with dehydroepiandrosterone (DHEA) for
PCOS modeling, with group D as the blank control injected with soybean oil. Vaginal smears and serological testing were
taken to assess the modeling. After the modeling, the rats in group A received 10 μg reorganized visfatin injection and those in
group B were treated with metformin (14 mg/100 g) on a daily basis for 15 days. Serum levels of T, LH, FSH, FINS and blood
glucose levels during OGTT were measured before and after the treatments, and HOMA-IR and LH to FSH ratio were
calculated. The ovaries were then dissected for pathological examination. Results In groups A and B, FINS, FPG, T, HOMA-IR
and blood glucose levels during OGTT were significantly decreased after the treatments (P<0.05), which resulted in recovery of
regular menses in 8 (80%) rats in group A and 7 (77.8%) rats in group B with the development of normal follicles. Visfatin and
metformin produced equivalent therapeutic effects in improving the insulin resistance and hyperandrogenism in PCOS rats.
Conclusion Visfatin and metformin have equivalent therapeutic effects in improving insulin resistance and hyperandrogenism
and in promoting the recovery of regular menses and development of normal follicles in PCOS rats.