高果糖诱导大鼠胰岛素抵抗模型的胰腺组织代谢组学研究

目的应用基于核磁共振氢谱（1H nuclear magnetic resonance, 1HNMR）的代谢组学方法研究高果糖诱导大鼠胰岛素抵抗
（insulin resistance, IR）模型的胰腺代谢组变化。方法选取Wistar大鼠16只，适应1周后随机分为2组：正常对照组和模型组，
每组8只。模型组给予10%果糖水喂饲8周，制备胰岛素抵抗模型；正常对照组给予等体积的纯净水。实验8周后取大鼠胰腺组
织，采集两组大鼠胰腺组织水溶性提取液的1H NMR谱，运用主成分分析法（principal component analysis, PCA）分析。结果与
正常对照组相比，肌酸、甜菜碱/氧化三甲胺、牛磺酸、甘氨酸和肌醇在模型组中是升高的，乳酸、胆碱和甘油磷脂胆碱/磷脂胆碱
则是降低的，且均具有显著性差异。结论基于核磁共振和模式识别的代谢组学方法能够给出胰岛素抵抗模型胰腺组织提取液
的代谢特征，为理解胰岛素抵抗状态下胰腺组织的代谢变化提供依据。

1H NMR metabonomics study of pancreatic extracts from insulin-resistant rats induced by fructose feeding

Objective To study the metabolic changes of pancreatic extracts from insulin-resistant rats induced by fructose
feeding using nuclear magnetic resonance 1H spectroscopy (1H NMR). Methods Sixteen Wistar rats were divided equally into
control group and model group and given water and 10% fructose water for 8 weeks, respectively. The pancreatic tissues were
then obtained for 1H NMR spectra analysis and principal component analysis (PCA). Results Compared with the control rats,
the rats in the model group showed significantly increased creatine, betaine/TMAO, taurine, glycine and myo-inositol and
decreased levels of lipids, lactate, glutamate, choline and GPC/PC. Conclusion 1H NMR and pattern recognition can define the
metabolic characteristics of the pancreatic tissue extracts from insulin-resistant rats and provide reliable metabolic evidence for
studying the mechanisms of insulin resistance at the molecular level.