| HPLC法测定五丹胃福颗粒中柚皮苷的含量 |
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| 引用本文： | 杨静,潘桂湘,王跃飞,辛丹.HPLC法测定五丹胃福颗粒中柚皮苷的含量[J].现代中药研究与实践,2009,23(2). |
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| 作者姓名： | 杨静 潘桂湘 王跃飞 辛丹 |
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| 作者单位： | 天津中医药大学,方剂学教育部重点实验室,天津,300193
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| 摘 要： | 目的建立五丹胃福颗粒中柚皮苷的HPLC含量测定方法。方法采用依利特HypersilODS2(4.6mm×250mm,5μm)色谱柱,乙腈-水(21:79)为流动相,流速1.0mL/min,紫外检测波长283nm,柱温25℃下对柚皮苷进行含量测定。结果柚皮苷的进样量在0.001128～0.061μg范围内与峰面积呈良好的线性关系,回归方程为:Y=1.7×106X-3.2×104,r=0.9999,平均回收率为98.60%,RSD为1.6%(n=6)。结论本法灵敏、简便、准确,重现性好,可用于五丹胃福颗粒中柚皮苷的含量测定。
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| 关 键 词： | 高效液相色谱法 五丹胃福颗粒 柚皮苷 |
Quantitative Determination of Naringin in Wudanweifu Granules by HPLC |
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| YANG Jing,PAN Gui-xiang,WANG Yue-fei,XIN Dan.Quantitative Determination of Naringin in Wudanweifu Granules by HPLC[J].Research and Practice on Chinese Medicines,2009,23(2). |
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| Authors: | YANG Jing PAN Gui-xiang WANG Yue-fei XIN Dan |
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| Abstract: | Objective To establish determination method of naringin in Wudanweifu granules by HPLC. Metheds HPLC analysis was performed on Hypersil ODS2 column (4.6 mm×250 mm, 5 μm). The mixture solution of acetonitrile-water (21:79) was used as the mobile phase. The flow rate was 1.0 mL/min. The detection wavelength was at 283 nm. The column temperature was maintained at 25 ℃ . Results Naringin had a good linearity in the range of 0.001 128～0.061 μg, the average recovery was 98.60% with RSD of 1.6%(n=6). Conclusion The method was sensitive, simple, accurate and reproducible, and suitable for the quality control for Wudanweifu granules. |
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| Keywords: | HPLC Wudanweifu granules naringin |
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