幽门弹簧插入配合高盐热糊灌胃复制大鼠慢性萎缩性胃炎癌前病变模型形态学观察及早期细胞凋亡分析

目的:研究幽门弹簧插入配合高盐热糊灌胃法复制慢性萎缩性胃炎(CAG)癌前病变大鼠模型形态学及早期细胞凋亡特征。方法:采用幽门弹簧插入配合高盐热糊灌胃法复制CAG癌前病变模型,用双标记流式细胞术分析CAG癌前病变大鼠细胞凋亡的特征。结果:光镜观察表明CAG癌前病变模型复制成功,CAG癌前病变模型组大鼠早期凋亡率明显高于正常组(P<0.01)。结论:幽门弹簧插入配合高盐热糊灌胃法成功复制CAG癌前病变大鼠模型,细胞凋亡增强可能是CAG癌前病变的发病机制之一。

Observation of pathologic picture and analysis of early apoptosis of precancerous lesion of CAG rats replicated by methods of phlorus spring insertion and hot paste

Objective: To study the feature of the pathologic picture and early apoptosis of precancerous lesion of chronic atrophic gastritis (CAG) rats replicated by methods of phlorus spring insertion and hot paste. Methods: Rats models were established by methods of phlorus spring insertion and hot paste, quantify the feature of the early apoptosis of precancerous lesion of chronic atrophic gastritis rats with annexin V FITC/PI double labeled flow cytometry. Results: Pathologic picture show the rats model of precancerous lesion of CAG is convincing .The ratio of the early apoptosis of the model control group increased moresignificantly than that of normal group (P<0.01). Conclusions: It is convincing to replicate the rat model of precancerous lesion of CAG by methods of phlorus spring insertion and hot paste. Increase in the apoptosis may be one of the etiopathogenesis of precancerous lesion of CAG.