悬浮培养的铁皮石斛原球茎在固体培养基上生长和分化的研究

目的:对悬浮培养的铁皮石斛原球茎在固体培养基上的生长和分化状况进行研究,以解决原球茎转接成活率低的问题。方法:选用不同基本培养基、碳源、培养基pH和激素,以生长鲜重和成苗数为指标,考察原球茎的生长和发育。结果与结论:降低固体基本培养基元素的含量有利于原球茎的生长,但不利于原球茎的分化。NAA,IAA,IBA和GA有利于原球茎的生长与分化,而偏酸的培养基pH和灭活的真菌不利于原球茎的生长与分化,灭活真菌容易引起原球茎的形态变异。

Studies on transplanting suspension-cultured protocorms of Dendrobium candidum onto solid culture medium

Objective: The suspension-cultured protocorms of Dendrobium candidum were transplanted on the solid culture medium for studying the factors influencing their differentiation and growth. Method: The growth and differentiation of protocorms were detected when different base-media, plant phytohormones, carbohydrates and pH were applied. Result and Conclusion: The diluted MS medium promotes the growth of protocorms but inhibite differentiation. The phytohormone NAA, IAA, IBA and GA are helpful to the growth and differentiation of protocorms, however, the low pH values and presence of autoclaved fungi have suppressive effects on protocorms. The fungi also induce morphological variation of protocorms.