卵巢浆液性癌中受体酪氨酸激酶EphB3蛋白表达及临床意义

目的探讨受体酪氨酸激酶EphB3蛋白在正常输卵管上皮组织、卵巢浆液性肿瘤组织和卵巢浆液性癌组织中的表达及其与卵巢浆液性癌临床病理特征的关系。方法采用免疫组化法检测9例正常输卵管、12例卵巢浆液性囊腺瘤和53例卵巢浆液性癌组织中EphB3蛋白的表达情况,进一步分析EphB3蛋白表达与卵巢癌临床病理特征的关系。结果正常输卵管上皮和卵巢良性肿瘤中EphB3蛋白的强阳性(+++)表达率分别为100.0%(9/9)、75.0%(9/12),明显高于卵巢浆液性癌的18.9%(10/53),差异有统计学意义(P<0.001)。EphB3蛋白表达与肿瘤分级(r=-0.606,P<0.001)和FIGO分期(r=-0.463,P<0.001)呈负相关,而与患者年龄、大网膜转移、腹腔转移和盆腔淋巴结转移无关(P>0.05)。结论 EphB3可能成为区分高、低级别浆液性癌的辅助指标,帮助卵巢浆液性癌病理诊断及制定治疗方案。

Expression of EphB3 in ovarian serous carcinoma and its clinical significance

Objective To explore the expression of receptor tyrosine kinase EphB3 in normal fallopian tube, ovarian serous benign tumor and ovarian serous carcinoma, and its relation to clinical pathological parameters of ovarian serous carcinoma.Methods Nine cases of normal fallopian tubes, 12 cases of benign tumors and 53 cases of ovarian serous carcinomas were collected.All tissues were buffered formalin fixed and paraffin embedded.EnVision immunohistochemistry was used to detect the expression of EphB3.The relationship between expression of EphB3 and clinical pathological parameters was analyzed.Results EphB3 was highly expressed in normal fallopian tube (100.0%) and benign tumor (75.0%) and reduced expression in ovarian serous carcinoma (18.9%) with statistical difference(P<0.001).Expression of EphB3 was negatively associated with tumor grade(r=-0.606, P<0.001)and FIGO stage (r=-0.463, P<0.001), and was not related to age, metastasis of greater omentum, metastasis of peritoneal cavity and lymph node metastasis of pelvic cavity.Conclusion EphB3 may be used to distinguish low and high grade tumor, and benefit for diagnosis and therapy of ovarian carcinoma.