DAB2IP基因及蛋白在结直肠癌组织中的表达研究

目的 从mRNA和蛋白两个水平上探讨DAB2IP 在结直肠癌组织中的表达及意义。方法 应用核酸原位杂交和免疫组织化学两种方法分别检测127例结直肠癌组织和36例癌旁正常黏膜组织中DAB2IP的表达情况，分析DAB2IP基因及其蛋白表达与结直肠癌淋巴结转移、分化程度及Dukes分期等临床病理特征的关系。结果 结直肠癌组织中DAB2IP mRNA与蛋白的阳性表达率分别为85.04％、79.53％，低于癌旁正常组织的100.00％、94.45％，差异有统计学意义（P均＜0.001）。高、中分化到低分化结直肠癌组织中，DAB2IP的阳性表达率均逐渐下调，3组间差异有统计学意义 （P＜0.01）。结直肠癌无淋巴结转移组中的DAB2IP阳性表达率高于淋巴结转移组，且其mRNA与蛋白表达水平与淋巴结转移呈负相关（r=-0.2361，r=-0.3060；P均＜0.01）。DAB2IP的表达水平与Dukes分期有关，从A期到D期DAB2IP表达逐渐下调，差异有统计学意义（P＜0.01）； 但DAB2IP表达与结直肠癌患者的年龄、性别、发生部位及肿瘤大小均无关（P＞0.05）。结论 DAB2IP在结直肠癌的发生、发展中具有重要作用，且其表达水平与淋巴结转移呈负相关，可作为预测结直肠癌转移潜能及判断预后的一个重要指标。

Study on the expression of DAB2IP mRNA and protein in colorectal carcinoma

Objective To investigate the expression and significance of DAB2IP in colorectal carcinoma in mRNA and protein levels.Methods One hundred and twenty-seven cases of colorectal carcinoma tissues and 36 cases of adjacent normal colorectal tissues were examined by immunohistochemistry and nucleic acid in situ hybridization.The relationship between the expression of DAB2IP mRNA and protein with clinicopathological characteristics such as lymph node metastasis,differentiation grades and Dukes stages in colorectal carcinoma.Results The positive expression rate of DAB2IP mRNA and protein in colorectal carcinoma tissues was 85.04％,79.53％,lower than 100.00％,94.45％ in adjacent normal tissues with significant difference (all P＜0.001).DAB2IP was gradual high-expression in high,medium and low differentiative groups,and there was significant difference in three groups (P＜0.01).The positive expression rate of DAB2IP in non-lymph node metastasis group was significantly higher than that in lymph node metastasis group,and there were negatively related (r =-0.2361,r=-0.3060;P＜0.01).DAB2IP was gradual down-expression from Dukes A to Dukes D stages (P＜0.01).The expression of DAB2IP was not related to age,sex,tumor sitc,and tumor volume of colorectal carcinoma(P＞0.05).Conclusion DAB2IP has significant effect in the occurrence and development of colotretal carcinoma.The down-expression of DAB2IP is closely related to metastasis in colorectal carcinoma,which is a potentially important indication for the biological behaviors and prognosis of colorectal carcinoma.