非小细胞肺癌组织中miR-449a／b／c的表达及临床意义

目的 探讨非小细胞肺癌（NSCLC）组织中微小RNA（miRNA）-449a/b/c的表达水平、临床意义及与预后的关系。方法 收集2013年1月至2015年12月收治的82例手术切除的NSCLC组织及69例癌旁组织标本，采用荧光实时定量PCR（QPCR）法检测以上组织中miR-449a/b/c的表达并比较3者在NSCLC组织及癌旁组织中的分布差异，Pearson相关分析NSCLC组织中miR-449a、miR-449b和miR-449c表达的相关性，分析NSCLC组织中miR-449a/b/c表达与临床病理特征（性别、年龄、肿瘤大小、TNM分期、组织学类型和淋巴结转移）的关系，同时根据随访数据比较不同miR-449a/b/c表达患者的预后情况。结果 QPCR结果显示，NSCLC组织中miR-449a、miR-449b和miR-449c的平均表达量依次为0.210±0.028、0.359±0.031和0.133±0.020，均低于癌旁组织，差异有统计学意义（P＜0.05）；NSCLC组织中miR-449a、miR-449b和miR-449c的表达均呈正相关，相关系数rmiR-449a/b、rmiR-449a/c和rmiR-449b/c分别为0.246、0.390和0.331（P＜0.05）；NSCLC组织中miR-449a/b/c表达均与TNM分期有关，miR-449a和miR-449c表达与肿瘤大小有关。全组中位总生存期（OS）为12.4个月，其中miR-449a低表达组和高表达组的中位OS分别为11.2个月和13.5个月（P＞0.05），miR-449b低表达组和高表达组的中位OS分别为9.6个月和14.2个月（P＜0.05），miR-449c低表达组和高表达组的中位OS分别为11.7个月和13.7个月（P＞0.05）。结论 miR-449a/b/c在NSCLC组织中表达降低，且均与TNM分期有关，miR-449b表达与预后有关，可能与NSCLC发生、发展有关，对NSCLC诊断及病情评估有一定价值。

Analysis of miR-449a/b/c expressions and their clinical significance in non-small cell lung cancer

Objective To investigate the expression and clinical significance of miR-449a/b/c in non-small cell lung cancer (NSCLC) as well as their expressions with prognosis.Methods We collected 82 cases of surgically resected NSCLC tissues and 69 cases of adjacent normal tissue (>5 cm away from cancer tissue) from January 2013 to December 2015.The expressions of miR-449a/b/c of above tissues were detected by using real-time fluorescence quantitative PCR (QPCR).The differences of distribution of miR-449a/b/c expressions in NSCLC and adjacent normal tissues were compared.Pearson correlation analysis of miR-449a, miR-449b and miR-449c in NSCLC tissues was undergone.The relationship of miR-449a/b/c expressions in NSCLC with clinical pathological parameters (gender, age, tumor size, TNM stage, histological type and lymph node metastasis) were analyzed.According to the follow-up data, the prognosis of different expressions of miR-449a/b/c was compared.Results QPCR results showed that in NSCLC tissues, the avarage expressions of miR-449a, miR-449b and miR-449c were 0.210±0.028, 0.359±0.031 and 0.133±0.020, lower than those in adjacent normal tissues, and the differences were statistically significant (P<0.05).The expressions of miR-449a, miR-449b and miR-449c were positively correlated in NSCLC and correlation coefficients for miR-449a/b, miR-449a/c and miR-449b/c were 0.246, 0.390 and 0.331 (P<0.05).The miR-449a/b/c levels of NSCLC tissues were significantly associated with TNM stage, and miR-449a and miR-449c were related to tumor size.All patients were followed up, and the median overall survival (OS) was 12.4 months.The median OS for miR-449a low expression group and high expression group were 11.2 and 13.5 months (P>0.05).The median OS for miR-449b low expression group and high expression group were 9.6 and 14.2 months (P<0.05).The median OS for miR-449c low expression group and high expression group were 11.7 and 13.7 months (P>0.05).Conclusion The expression of miR-449a/b/c in NSCLC tissues was decreased, and all of them were related to TNM stage.The expression of miR-449b was related to prognosis, which may be related to the occurrence and development of NSCLC.It has some value in the diagnosis and evaluation of NSCLC.