卵巢浆液性／黏液性囊腺癌中SP1、KLF9的表达及与HPV16／18感染的相关性研究

目的 探讨KLF9、SP1及HPV16／18在卵巢浆液性／黏液性囊腺癌中的表达及临床意义，并进一步探讨三者在浆液性／黏液性卵巢癌发生、发展过程中的作用。方法 制作组织芯片并采用免疫组化SP法及分子原位杂交技术检测60例卵巢浆液性／黏液囊腺癌组织及20例卵巢浆液性／黏液性囊腺瘤组织中KLF9、SP1和HPV16／18的表达情况，分析三者与临床病理特征的关系，并检验三者之间的相关性。结果 SP1、KLF9及HPV16／18在卵巢浆液性／黏液性囊腺癌组织中的阳性表达率分别为71.7％（43／60）、63.3％（38／60）、30.0％（18／60），而三者在卵巢浆液性／黏液性囊腺瘤组织中分别为15.0％（3／20）、10.0％（2／20）、0（0／20），差异均有统计学意义（P＜0.01）。KLF9表达与各临床病理特征均无关（P＞0.05）；SP1表达与临床分期有关（P＜0.05）；HPV16／18表达与分化程度有关（P＜0.05）。在卵巢浆液性／黏液性囊腺癌组织中KLF9与SP1的表达呈正相关（r=0.443，P=0.000），HPV16／18与SP1的表达亦呈正相关（r=0.331，P=0.010），KLF9与HPV16／18的表达无相关性（P=0.133）。结论 KLF9、SP1及 HPV16／18在卵巢浆液性／黏液性囊腺癌组织中高表达，可能与卵巢浆液性／黏液性囊腺癌的生物学行为有关，在浆液性／黏液性卵巢癌的发生、发展中起重要的促进作用。

Expression and significance of KLF9,SP1 and HPV16/18 in serous or mucinous ovarian cystadenocarcinoma

Objective To investigate the expressions of KLF9, SP1 and HPV16/18 protein in ovarian serous or mucinous cystadenocarcinoma and their clinicopathological significance, and further investigate the effect of KLF9, SP1 and HPV16/18 on the occurrence and development of ovarian serous or mucinous cystadenocarcinoma.Methods Sixty cases of ovarian serous or mucinous mucinous cystadenocarcinoma and 20 cases of ovarian serous or mucinous cystadenoma were collected in this study.KLF9, SP1 and HPV16/18 were detected by immunohistochemical SP method and in situ hybridization.We also analyzed the relationship of their expression and clinicopathological features as well as the correlation among them.Results The positive rates of SP1, KLF9 and HPV16/18 were 71.7%(43/60), 63.3%(38/60)and 30.0%(18/60) in ovarian serous or mucinous cystadenocarcinoma, higher than 15%(3/20), 10%(2/20)and 0(0/20)in ovarian serous or mucinous cystadenoma (P<0.05).Spearman correlation showed that there was significantly positive correlation between KLF9 and SP1(r=0.443,P=0.000), and also positive correlation between HPV16/18 and SP1(r=0.331,P=0.010).The expression of KLF9 was not correlated with any clinicopathological features (P>0.05).The expression rate of SP1 was significantly correlated with clinical stage (P<0.05).The expression of HPV16/18 was correlated with pathological grade (P<0.05).Conclusion KLF9, SP1 and HPV16/18 were highly expressed and may affect the biological behavior of ovarian serous or mucinous cystadenocarcinoma.They may play an important role in the occurrence and development of ovarian serous or mucinous cystadenocarcinoma.