肺癌患者外周血CD4^＋CD25^＋调节性T细胞的检测及意义

目的研究肺癌患者外周血CD4^＋CD25^＋调节性T细胞（Treg）的比例变化，探讨其与肺癌的临床分期、病理类型及组织学分化程度的相关性。方法采用流式细胞术检测78例肺癌患者（肺癌组）和30例健康对照者（健康对照组）外周血CD4^＋CD25^＋Treg占CD4^＋T细胞的比例。结果肺癌组外周血CD4^＋CD25^＋Treg占CD4’T细胞的比例为（21．07±5．26）％，与健康对照组（10．04±2．75）％比较，差异有统计学意义（P〈0．05）；Ⅲ、Ⅳ期肺癌患者外周血CD4^＋CD25^＋Treg比例分别为（22．46±4．35）％、（24．35±6．20）％，显著高于Ⅰ期＋Ⅱ期肺癌患者（12．89±3．19）％及健康对照组（10．04±2．75）％相比，差异有统计学意义（P〈0．05）；鳞癌、腺癌、小细胞肺癌患者外周血CD4^＋CD25^＋Treg比例分别为（20．75±4．85）％、（21．52±5．26）％、（19．20±6．41）％，与健康对照组比较，差异均有统计学意义（P〈0．05），但不同病理类型的肺癌患者间外周血CD4^＋CD25^＋Treg比较，差异无统计学意义（P〉0．05）；高分化、中分化、低分化肺癌患者外周血CD4^＋CD25^＋Treg比例分别为（20．36±3．12）％、（21．11±4．67）％、（21．30±5．75）％，显著高于对照组（P〈0．05），但不同分化程度的各肺癌者间比较，差异无统计学意义（P〉0．05）。结论肺癌患者外周血CD4^＋CD25^＋Treg水平明显升高，且与肺癌的进展密切相关，但与病理类型、组织学分化程度无关。

The detection and significance of CD4+CD25+ regulatory T cells in peripheral blood of patients with lung cancer

Objective To evaluate the proportion of CD4+CD25+regulatory T cells(Treg)in peripheral blood of patients with lung cancer and observe the relationship of clinical stage,pathologic types,relation between CD4+CD25+ Treg and lung cancer.Methods Flow cytometer was used to analyze the level of CD4+CD25+ Treg in peripheral blood from seventy-eight cases of lung cancer patients and 30 cases of healthy donors.Results The proportion of CD4+CD25+ Treg in peripheral blood was significantly higher in lung cancer patients(21.07±5.26)%when compared with healthy donors(10.04±2.75)%(P＜0.05).The proportion of CD4+CD25+ Treg was significantly higher in stage Ⅲ or Ⅳ lung cancer patients(22.46±4.35)%,(24.35±6.20)% than in stage Ⅰ and Ⅱ lung cancer patients(12.89±3.19)% and healthy donors(P＜0.05).The proportion of CD4+CD25+ Treg was significantly higher in squamous cell carcinoma(20.75±4.85)%,adenocarcinoma(21.52±5.26)% and small cell lung cancer patients(19.20±6.41)% when compared with healthy donors(P＜0.05)but without significance among each teams(P＞0.05).The proportion of CD4+CD25+ Treg was significantly higher in high differentiation (20.36±3.12)%,middle differentiation(21.11±4.67)% and low differentiation(21.30±5.75)% lung cancer patients than in healthy donors(P＜0.05)but without significance among each teams(P＞0.05).Conclusion The proportion of CD4+CD25+ Treg in the peripheral blood of patients with lung cancer is significantly higher than healthy donors.It plays an importmant role in the progression of lung cancer.The up-regulation of CD4+CD25+Treg is not related with pathologic types.