SLE患者外周血单个核细胞bcl-2和bax基因的表达

目的通过对系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)凋亡相关基因bcl-2与bax mRNA的检测,探讨凋亡相关基因在SLE患者中的改变及其在SLE发病机理中的作用。方法应用逆转录-PCR(RT-PCR)法检测41例SLE患者及32例年龄、性别与之相匹配的正常人PBMC中bcl-2mRNA与bax mRNA的表达,并用免疫印迹法检测SLE患者组自身抗体谱。结果SLE活动期患者组bcl-2mRNA表达明显高于稳定期患者和正常对照组,稳定期患者和正常对照组比较也有显著性升高;SLE患者中,两种以上自身抗体阳性组bcl-2 mRNA表达与一种或无自身抗体阳性组相比显著升高;各受试组bax mRNA的表达均无显著性差异。结论SLE患者PBMCbcl-2 mRNA呈过度表达,而baxmR-NA的表达无变化,使得bcl-2/bax的比值升高,这可能是大量自身抗体产生,SLE发生发展的重要因素之一。

Study on the Levels of Expression of mRNA of bcl-2 and bax in PBMC of Patients with SLE

Objective To determine the expression of mRNA of bel-2 and bax in human SLE patients and inquire into the roles of which in the pathogenesis of SLE. Methods The levels of the expression of mRNA of bcl-2 and bax of 41 SLE patients and control group of 32 healthy persons which age and sex-matched were detected by RT-PCR;While anti-ENA autoantibodies of SLE patients were detected with Western-blot, Results The levels of expression of mRNA of bcl-2 of active SLE patients was significantly higher than that of the quiescent SLE patients, and of the normal healthy donors, and that of quiescent SLE patients was significantly higher than that of control groups, Statistically significant differences were shown between, one group with two and more kinds of autoantibodies,and another group which had at most one kind of autoantibody in SLE patitents, Conclusion The levels of mRNA of bcl-2 of SLE patients significantly increased perhaps palys an important role of occurance and development of human SLE.