Mucosal inflammatory cytokine production by intestinal biopsies in patients with ulcerative colitis and Crohn's disease |
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| Authors: | J. -M. Reimund C. Wittersheim S. Dumont C. D. Muller R. Baumann P. Poindron B. Duclos |
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| Affiliation: | (1) Service d'Hépatogastroenté rologie et d'Assistance Nutritive, CHRU Hautepierre, 67098 Strasbourg Cédex, France;(2) Département d'Immunologie, Immunopharmacologie et Pathologie, Centre de Recherches Pharmaceutiques, 67401 Illkirch-Graffenstaden, France;(3) Unité de Recherche INSERM U381, Avenue Molière, 67000 Strasbourg Cédex, France;(4) Laboratoire de Biophysique, URA 491 du CNRS, Centre de Recherches Pharmaceutiques, 67401 Illkirch-Graffenstaden, France;(5) Present address: Département d'Immunologie, Immunopharmacologie et Pathologie, Centre de Recherches Pharmaceutiques, 74 Route du Rhin, 67401 Illkirch-Graffenstaden, France |
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| Abstract: | Chronic inflammation in inflammatory bowel disease (IBD; Crohn's disease and ulcerative colitis) may be attributed partly to increased secretion of inflammatory cytokines. The aim of this study was to investigate simultaneously the spontaneous release patterns of tumor necrosis factor-alpha (TNF- ), interleukin-1-beta (IL-1 ), and interleukin-6 (IL-6) by organ cultures of inflamed mucosa from IBD patients. Organ cultures of involved IBD mucosa spontaneously produced increased amounts of TNF-, IL-1, and IL-6 compared to normal mucosa. The patterns of cytokine release between Crohn's disease and ulcerative colitis organ cultures were not significantly different. Increased inflammatory cytokine production by lamina propria mononuclear cells (LPMCs) and mucosa treated with EDTA suggests that these cytokines originate mainly from LPMCs. These results confirm the role of inflammatory cytokines in IBD and shed a new light on the role of TNF- in IBD. |
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| Keywords: | Crohn's disease ulcerative colitis cytokines organ cultures inflammation |
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