PCR-SBT法HLA基因分型模棱两可结果的判读及解决对策

目的研究PCR—SBT法HLA基因分型结果判读中出现的模棱两可问题并提出解决策略。方法对306名随机抽取的组织配型患者的DNA采用PCR-序列特异性引物（Sequence Specific Primer，SSP）低分辨方法检测，同时采用PCR—SBT法进行HLA—A，B，DRB1高分辨基因分型。PCR—SBT法模棱两可结果用PCR—SSP高分辨方法复核确认。结果所有306例检测标本的高低分辨血清学抗原一致，经PCR—SBT基因测序方法检测有111份模棱两可结果，占36．3％（111／306）。其中HLA—A座位有30对等位基因存在模棱两可结果，占所有检测标本等位基因总数的3．3％；HLA—B座位有66对，占7．2％；HLA—DRB1座位有36对，占3．9％。所有模棱两可标本经PCR—SSP HLA基因高分辨分型试剂PCR—SSP等方法复核确认。结论针对PCR—SBT法进行HLA—A，B，DRB1高分辨基因分型模棱两可结果所建立的解决策略，对于提高HLA数据分型的速度和分型准确性有重要意义。

Judgment on the Ambiguous Results of PCR-SBT HLA Genotyping and its Solution

Objective To investigate the frequencies of HLA-alleles ambiguous typings by sequence based typing and propose the way to resolve.Method Of 306 randomly selected patients,their DNAs were genotyped using PCR-sequence specific primer(SSP) for low resolution typing and PCR-SBT for allelic designation of HLA-A,B,DRB1.PCR-SSP high-resolution kit were used to resolve the PCR-SBT ambiguous typings.Results Each of the 306 samples showed the identical serological antigens for both low and high resolution genotyping ...