鹿茸多肽对四氯化碳所致小鼠急性肝损伤的保护作用

 目的观察梅花鹿茸多肽(VAP)对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法腹腔注射四氯化碳制备小鼠急性肝损伤模型,检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性和白蛋白(ALB)含量;同时测定肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷光甘肽(GSH)的活性。在光学显微镜下观察肝脏病理组织学变化。结果与四氯化碳模型组比较,VAP可使血清ALT,AST活性明显降低(P<0.01),但对ALB的含量无明显影响(P>0.05);同时VAP可使肝组织中MDA的含量明显下降(P<0.01),GSH含量有所升高,SOD活力明显增强(P<0.01)。形态学观察,VAP能明显改善肝组织的病理学改变。结论VAP对四氯化碳所致小鼠急性肝损伤具有保护作用。

Protective Effect of Velvet Antler Peptides on Acute Hepatic Injury by Carbon Tetrachloride

OBJECTIVE To study the protective effects of velvet antler polypeptides(VAP) on acute hepatic injury induced by carbon tetrachloride(CCl₄) in mice.METHODS Sixty Kunming mice were randomly divided into 6 groups.The VAP groups were treated with VAP at a dose of 12.5,25 and 50 mg·kg^-1·d^-1 for 5 days subcutaneously.At the same time,the normal control group and the model control group were given equivalent amount of 0.9% sodium chloride solution by the same route.And mice in the positive control group were intragastically administrated with 200 mg·kg^-1·d^-1 BDD.After the last administration,all groups except the normal control were intraperitoneally injected with 0.2% CCl₄ solution to induce acute hepatic injury.Thereafter, the contents of ALT,AST,ALB in the serum,MDA and the activity of SOD,GSH in the liver were measured and the hepatic histological changes were observed by optical microscope.RESULTS Compared with the model group, the contents of ALT,AST in the serum was obviously decreased(P<0.01,P<0.001),the content of MDA in the liver was also content lowered(P<0.01),and the activity of GSH and SOD in the liver was markedly improved(P<0.05,P<0.01) after preventive treatment with VAP.VAP Ameliorated the hepatic pathological changes.CONCLUSION VAP have protective effects on acute hepatic injury induced by CCl₄ in mice.