FQ—PCR血、尿、乳汁联合检测在小儿人巨细胞病毒感染中的临床研究

目的探讨用荧光定量聚合酶链反应（fluorescence quantitative PCR，FQ—PCR）法进行血、尿、母乳联合检测小儿人巨细胞病毒（HCMV）的临床意义。方法采用FQ—PCR法联合检测182例疑诊HCMV感染的患儿，对母乳喂养的阳性患儿补测母乳HCMV—DNA。结果血、尿及联合检测HCMV—DNA阳性率分别为15．93％、29．12％、39．01％。阳性患儿配对的母乳HCMV—DNA阳性率为45．00％，阴性患儿配对的母乳HCMV—DNA阳性率为18．60％，两组进行x^2检验，x^2=5．54，具有显著性差异（P〈0．02）。结论联合检测能提高HCMV—DNA检测阳性率，尿HCMV—DNA检测阳性率高于血HCMV—DNA检测，HCMV感染母乳是婴儿获得性感染的主要途径之一。

Clinical study of application fluorescent quantitative PCR on diagnosis of HCMV infection in children

Objective： To understand the clinical significance of HCMV combined testing of serum, urine, breast milk samples using fluorescent quantitative polymerase chain reaction （FQ -PCR） in children. Methods： HCMV -DNA in serum and urine was detected by using FQ -PCR in 182 suspect cases, as well as breast milk from mothers, whose children was feed by breast and its HCMV infection was confirmed. Results： The positive rate of HCMV -DNA of serum, urine and combined testing was 15. 93%, 29. 12%, 39. 01%, respectively. The HCMV-DNA positive rate of breast milk from confirmed case＇s mother was significant higher than that of control group （X^2 = 5.54, P 〈 0. 02）. Conclusion： The results indicated that combined testing could increase the positive rate of HCMV - DNA, the HCMV - DNA positive rate of urine was higher than serum, and breast milk infected with HCMV should be the main route of acquired infection.