造血干细胞联合AGM基质细胞移植对骨髓移植小鼠造血重建的影响

目的探讨造血干细胞与主动脉-性腺-中肾(Aorta-gonad-mesonephros,AGM)区来源的基质细胞联合移植对同基因骨髓移植(bone marrow transplantation,BMT)小鼠BMT后骨髓造血的影响。方法建立同基因骨髓移植小鼠模型,随机分成4组:空白对照组、BMT组、BMT联合AGM基质细胞移植组(联合移植组)及川芎嗪组,另设正常组。分别于BMT后第7、14、21、28d检测外周血细胞、骨髓单个核细胞(bone marrow mono-nuclear cells,BMMNC)、第3、7、10、14、 21、28d检测骨髓组织学变化。结果联合移植组骨髓单个核细胞较川芎嗪组恢复快,联合移植组及川芎嗪组外周血血细胞、骨髓单个核细胞、骨髓组织恢复均较单纯BMT组快,有显著性差异。结论 BMT联合AGM基质细胞移植对骨髓移植造血重建具有促进作用。

Effect of Cotransplantation of Hematopoietic Stem Cells and Embryonic AGM Stromal Cells on Hematopoietic Reconstitution in Mice after Bone Marrow Transplantation

Objective To explore the effects of cotransplantation of hematopoietic stem cells and stromal cells derived from aorta-gonad-mesonephros (AGM) region on hematopoietic reconstitution in mice after bone marrow transplantation.Methods The typical model of syngeneic BMT was established and the model mice were randomly divided into 4 groups:the control group,the BMT group,the group of cotransplantation of HSC with AGM stromal cells (the cotransplantation group) and the ligustrazine group (the LT group).The following factors were measured on day 3,7,10,14,21 and 28 after BMT:peripheral blood cells,bone marrow mononuclear cells (BMMNC), and histology changes of bone marrow. Results The levels of periphral WBC,RBC,platelet,and BMMNC in the contransplantation group were higher than those in the single BMT group and the LT group.Conchision Cotransplantation with AGM stromal cells could significantly promote hematopoietic reconstruction in mice after BMT.Cotransplantation may represent a promising means of achieving higher engraftment rate after BMT.