高效液相色谱法同时测定海珠喘息定片中槲皮素和山柰酚含量

目的 建立同时测定海珠喘息定片中主药胡颓子叶的指标性成分槲皮素和山柰酚含量的高效液相色谱法.方法 色谱柱为Shimadzu Shim-pack GIST-C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.4％磷酸水溶液(50:50,V/V),流速为1.0 mL/min,柱温为30℃,检测波长为360 n...

Simultaneous Determination of Quercetin and Kaempferol in Haizhu Chuanxiding Tablets by HPLC

Objective To establish a high-performance liquid chromatography(HPLC)method for simultaneous determination of quercetin and kaempferol in Haizhu Chuanxiding Tablets.Methods The chromatographic column was Shimadzu Shim-pack GIST-C₁₈column(250 mm×4.6 mm,5μm),the mobile phase was methanol-04%phosphoric acid solution(50∶50,V/V),the flow rate was 1.0 mL/min,the column temperature was 30℃,the detection wavelength was 360 nm,and the injection volume was 20μL.Results The linear ranges of quercetin and kaempferol were 0.43-8.59μg/mL(R²=0.9997,n=9)and 0.30-5.91μg/mL(R²=0.9997,n=9),respectively.The limits of detection(LOD)were 0.43 ng and 0.59 ng,respectively,and the limits of quantitation(LOQ)were 1.29 ng and 1.77 ng,respectively.The RSDs of precision,stability and repeatability tests were less than 2.0%.The average recoveries of quercetin and kaempferol were 101.67%and 101.36%with RSDs of 1.54%and 1.99%(n=9),respectively.Conclusion The method is simple,accurate,specific,reproducible and stable,which can be used for the simultaneous determination of quercetin and kaempferol in Haizhu Chuangxiding Tablets.