不同酶联免疫吸附法检测丙肝抗体的效果

目的:研究用不同酶联免疫吸附法（enzyme-linked immunosorbent assay，ELISA）检测丙型肝炎（简称丙肝）抗体的效果。方法:选取深圳市罗湖区辖区医院2019年6月至2021年6月收治的20～65岁男性80例丙肝患者血清，根据检测方式分为常规组（间接ELISA法，40例）和试验组血清（双抗原...

Effects of different enzyme-linked immunosorbent assays in detecting hepatitis C antibody

Objective　To study the effects of different enzyme-linked immunosorbent assays(ELISA) in detecting hepatitis C antibody. Methods　Eighty male hepatitis C patients who were 20-65 years old and who wereadmitted to hospitals at Luohu District from June 2019 to June 2021 were selected.According to the detection methods, they were divided into a conventional group(40 cases), detected by the indirect ELISA, and an experimental group (40cases), detected by the double antigen sandwich ELISA. The test results of thetwo groups were compared and analyzed. The independent-sample t and χ² test were applied. Results　There were 8 anti-viral hepatitis C (HCV) positive samples, and thepositive detection rate was 87.50% (7/8) in the experimental group, and was25.00% (2/8) in the conventional group; there were 72 anti-HCV negativesamples, and the negative detection rate was 97.22% (70/72) in the experimentalgroup, and was 83.33% (60/72) in the conventional group; there were statisticaldifferences between these two groups (χ²=6.349 2 and 7.912 1; P=0.0117 and 0.004 9). The sensitivity, specificity, and positive and negativepredictive values in the experimental group were higher than those in theconventional group, with statistical differences (all P<0.05). Conclusion　The double antigensandwich ELISA has high specificity and sensitivity in the detection ofhepatitis C antibody, so it can provide reference data for disease treatmentand prognosis improvement, and is worth being generalized.