| 1，6－二磷酸果糖对培养心肌细胞膜缺氧－再灌注损伤的保护作用 |
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| 引用本文： | 黄乃祥,叶世铎.1，6－二磷酸果糖对培养心肌细胞膜缺氧－再灌注损伤的保护作用[J].心肺血管病杂志,1995(4). |
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| 作者姓名： | 黄乃祥 叶世铎 |
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| 作者单位： | 同济医科大学心血管疾病研究所外科 |
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| 摘 要： | 用培养的原代心肌细胞建立缺氧－再给氧方法，对缺氧６０分钟、１２０分钟及缺氧后再给氧３０及６０分钟时心肌细胞搏动、ＬＤＨ活性、（５１）￣Ｃｒ释放率、苔盼蓝摄取率、扫描电镜观察及１，６－二磷酸果糖对其影响进行了研究。结果显示：缺氧２小时可引起心肌细胞搏动频率减慢，再给氧后搏动频率加快；ＬＤＨ活性、（５１）￣Ｃｒ释放率和苔盼蓝摄取率增加，电镜显示心肌细胞膜受损。１，６－二磷酸果糖对心肌细胞膜具有保护作用。
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| 关 键 词： | 1，6－二磷酸果糖，培养心肌细胞，心肌再灌注损伤 |
Protective Effects of Fructose-1,6-Diphosphate on Cultured Myocardial Cell Membrane with Anoxia/Reperfusion Injuries |
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| Huang Naixiang and Ye Shiduo.Protective Effects of Fructose-1,6-Diphosphate on Cultured Myocardial Cell Membrane with Anoxia/Reperfusion Injuries[J].Journal of Cardiovascular and Pulmonary Diseases,1995(4). |
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| Authors: | Huang Naixiang and Ye Shiduo |
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| Abstract: | In the present experiment an anoxia/reoxygenation method was established by using cultured primordial myocardial cells.The pulsation of myocardial cell, activity of LDH,release rate of 51Cr, uptake rate of trypan-blue and results of scanning electron microscopy were examined at 60 and 120 min after anoxia, 30 and 60 min after reoxygenation. The effects of FDP on the anoxia/reoxygenation myocardial cell were studied. The results showed that the beating of myocardial cells was slowed down, release rate of 51Cr,activity of LDH and uptake rate of trypan-blue was increased 2 hours after anoxia and 1 hour after reoxygenation. Electron microscopy revealed injury of cell membrane. FDP had some protective effects on myocardium during the anoxia/reoxygenation |
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| Keywords: | FDP Cultured myocardial cell Reperfusion injury of myocardium |
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