| 三氯乙酸沉淀法结合双缩脲比色法测定水蛭提取液中总蛋白含量 |
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| 引用本文： | 余兰,于香安. 三氯乙酸沉淀法结合双缩脲比色法测定水蛭提取液中总蛋白含量[J]. 中国药物与临床, 2004, 4(9): 685-686 |
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| 作者姓名： | 余兰 于香安 |
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| 作者单位： | 兰州医学院第二附属医院临床药理基地,730000 |
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| 摘 要： | 目 的 建 立水 蛭提 取 液中 蛋白 含 量的 快速 测 定方 法。方法 采用 三氯 乙 酸沉 淀法 结 合双 缩 脲比色 法测 蛋白 含 量，并做 加样 回收 率 试验 。结 果 平 均回 收率 为９９．３％ ，相对 标 准偏 差（ＲＳＤ ）为１．４％ 。结 论 该法 简便 、快 速、准 确 可靠 。
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| 关 键 词： | 三氯乙酸 双缩脲比色法 水蛭提取液 含量测定 |
| 修稿时间： | 2004-06-14 |
Trichloroacetic acid precipitation plus biuret colorimetry to determine total protein in hirudo extract |
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| YU Lan,YU Xiang-an. Trichloroacetic acid precipitation plus biuret colorimetry to determine total protein in hirudo extract[J]. Chinese Remedies & Clinics, 2004, 4(9): 685-686 |
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| Authors: | YU Lan YU Xiang-an |
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| Affiliation: | YU Lan,YU Xiang-an. The State Food and Drug Administration of the Second Affiliated Hospital of Lanzhou Medical College,Gansu 730000,China |
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| Abstract: | Objective To establish a fast method for the content determination of protein in extract from hir-udo. Method Uusing biuret colorimetry to determine the content of protein which had been precipitated by trichloroacetic acid. The average recovery test was also conducted. Result The mean recovery was 99.3% (RSD=1.4%). Conclusion The method is simple,rapid,and feasible. |
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| Keywords: | Trichloroacetic acid Biuret colorimetry Extract from hirudo Content determination |
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