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| 氟桂利嗪对小鼠生精细胞T型Ca~(2+)通道的作用 | |
| 引用本文： | 王昌松,高晓华,程宏,龚伟,张习春,程洁,高蓉,肖杭. 氟桂利嗪对小鼠生精细胞T型Ca~(2+)通道的作用[J]. 中华男科学杂志, 2006, 12(7): 594-597,601 |
| 作者姓名： | 王昌松 高晓华 程宏 龚伟 张习春 程洁 高蓉 肖杭 |
| 作者单位： | 1. 南京医科大学应用毒理省重点实验室,江苏,南京,210029 2. 江苏省疾病控制中心,江苏,南京,210009 3. 复旦大学神经生物学系,上海,200433 |
| 基金项目： | 国家自然科学基金;国家重点基础研究发展计划(973计划) |
| 摘 要： | 目的:研究二苯烷基氨类衍生物氟桂利嗪(F lu)对小鼠生精细胞T型钙电流(ICaT)的影响。方法:采用急性机械分离的小鼠生精细胞,应用膜片钳全细胞记录技术,观察F lu对ICaT的影响。结果:钳制电位为-90 mV、刺激电压为-30 mV时,F lu(0.1、1、10、100μmol/L)可抑制小鼠生精细胞Ca2+电流,抑制率分别为(23.34±2.76)%、(46.04±3.52)%、(62.52±3.70)%、(73.52±3.12)%(P<0.05);F lu对T型Ca2+通道的半数最大抑制浓度为0.29μmol/L。结论:F lu对生精细胞T型Ca2+通道有阻断作用,呈浓度依赖性;Cav3.2是生精细胞T型Ca2+电流的主要贡献者。 |
| 关 键 词： | 氟桂利嗪 T型Ca2+通道 生精细胞 小鼠 |
| 文章编号： | 1009-3591（2006）07-0594-05 |
| 收稿时间： | 2005-10-14 |
| 修稿时间： | 2005-10-142006-01-19 |
Effects of Flunarizine on T-type Calcium Channels in Mouse Spermatogenic Cells |
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| WANG Chang-song,GAO Xiao-hua,CHENG Hong,GONG Wei,ZHANG Xi-chun,CHENG Jie,GAO Rong,XIAO Hang. Effects of Flunarizine on T-type Calcium Channels in Mouse Spermatogenic Cells[J]. National journal of andrology, 2006, 12(7): 594-597,601 | |
| Authors: | WANG Chang-song GAO Xiao-hua CHENG Hong GONG Wei ZHANG Xi-chun CHENG Jie GAO Rong XIAO Hang |
| Affiliation: | Institute of Applied Toxicology of Nanjing Medical University, Nanjing, Jiangsu 210029, China. |
| Abstract: | OBJECTIVE: To investigate the effects of Flunarizine (Flu) on T-type calcium currents (ICaT) in spermatogenic cells. METHODS: Ca2+ currents were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp technique and the effects of Flu on ICaT were observed. RESULTS: Flu of 0.1, 1, 10, 100 micromol/L inhibited ICaT in mouse spermatogenic cells significantly with the K50 value of 0.289 micromol/L (P < 0.05). With the holding potential at -90 mV and stimulating potential at -30 mV, the inhibition rates of Flu were (23.34 +/- 2.76)%, (46.04 +/- 3.52)%, (62.52 +/- 3.70)% and (73.52 +/- 3.12)%, respectively. CONCLUSION: Flu has significant inhibitory effects on ICaT in mouse spermatogenic cells, with concentration dependence. Ca(v)3.2 is the main contributor to T-type Ca2+ currents in spermatogenic cells. |
| Keywords: | Flunarizine T-type Ca~(2 )channel spermatogenic cell mouse |
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