2,3-吲哚醌对二甲基苯蒽诱导的大鼠乳腺癌的预防作用

目的探讨2,3-吲哚醌(ISA)对二甲基苯蒽(DMBA)诱导的大鼠乳腺癌的化学预防作用。方法♀SD大鼠随机分为空白对照组、模型组、ISA低、中、高剂量给药组,一次性皮下给予DMBA诱导大鼠乳腺癌模型发生,观察ISA对DMBA诱导的乳腺癌模型大鼠肿瘤抑制率,脏器指标,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)活性,肿瘤坏死因子α(TNF-α)浓度,肿瘤组织病理学检查结果。结果 ISA能推迟大鼠乳腺癌的发生时间,降低肿瘤发生率;与模型组相比,低、中、高剂量组大鼠的脾指数及胸腺指数升高;低剂量组GSH-Px及SOD均明显升高,而MDA活性降低(P<0.05);ISA低、高剂量组TNF-α浓度较模型组有明显降低。结论 ISA对DMBA诱发的大鼠乳腺癌有预防作用,其机制可能为:增强荷瘤大鼠免疫系统的抵抗力,清除血中氧化应激产生的氧自由基,抑制肿瘤细胞的增殖。

Chemopreventive effect of isatin against DMBA-induced mammary carcinoma in rats

Aim To investigate the chemopreventive effect of isatin(ISA) against 7,12-dimethylbenzanthracene(DMBA) induced mammary carcinoma in rats.Methods Sprague-Dawley rats were divided into 5 groups:control group,model group,ISA(25,50,100 mg·kg -1 ) groups.DMBA was given to induce mammary carcinoma.The incidence rate of mammary carcinoma,the index of the spleen and the thymus gland,the activities of superoxide dismutase(SOD) ,glutathione peroxidase(GSH-Px) ,malondialdhyde(MDA) and the tumor necrosis factor(TNF-α) in blood serum were observed.HE staining was used to examine the tumors.Results ISA could delay the occurrence of DMBA-induced mammary tumors,reduce the incidence of mammary carcinoma;compared with model group,the index of spleen and thymus gland of different levels of ISA doses was increased.The activity of GSH-Px and SOD in the low-dose group was increased significantly,while the MDA activity was decreased significantly(P<0.05) .The concentration of TNF-αin the low and high dose group was lower than that in the model group.Conclusions These results suggest that ISA can chemoprevent the mammary carcinoma induced by DMBA in rats.The mechanism may be:clearing the free radical oxygen produced by oxidative stress and inhibiting the proliferation of carcinoma cell lines.