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| 河蚌多糖提取优化工艺的研究 | |
| 引用本文： | 沈鸿,彭蕴茹,窦昌贵.河蚌多糖提取优化工艺的研究[J].中成药,2006,28(5):746-748. |
| 作者姓名： | 沈鸿 彭蕴茹 窦昌贵 |
| 作者单位： | 1. 南京脑科医院,江苏,南京,210029 2. 江苏省中医药研究所,江苏,南京,210038 3. 中国药科大学中医药研究所,江苏,南京,210038 |
| 摘 要： | 目的:对河蚌多糖的提取工艺进行优化,从而提高多糖的含量。方法:本文采用正交设计的方法,以硫酸蒽酮法测定总糖含量为评价指标,从提取温度、NaOH浓度、提取次数等因素入手,进行正交优化试验。结果:提取温度、NaOH浓度为有极显著意义的因素,提取次数对多糖的含量影响不大,综合考虑,最佳提取工艺为A1B2C1即在4℃时,NaOH浓度为5%,提取1次。结论:优选得到的工艺经济,简单、稳定、可行。 |
| 关 键 词： | 河蚌多糖 正交设计 硫酸蒽酮法 |
| 文章编号： | 1001-1528（2006）05-0746-03 |
| 收稿时间： | 2005-08-10 |
| 修稿时间： | 2005-08-10 |
Optimum extraction process of mussel polysaccharide |
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| SHEN Hong,PENG Yun-ru,DOU Chang-gui.Optimum extraction process of mussel polysaccharide[J].Chinese Traditional Patent Medicine,2006,28(5):746-748. | |
| Authors: | SHEN Hong PENG Yun-ru DOU Chang-gui |
| Institution: | 1. Nanjing Brain Hospital, Nanjing 210029; 2. Jingsu Province Traditional Chinese Medicine Institute, Nanjing 210038; 3. Traditional Chinese Medicine Institute China Pharmaceutical University, Nanjing 210038, China |
| Abstract: | AIM: To: optimize the extraction process of mussel polysaccharide, and then gain higher content of polysaccharide. METHODS: Involved in the three factors, temperature and times of extraction, concentration of NaOH solutions, to do the experiment by orthogonal design uniform design and the method of anthrone. RESULTS: The result showed that the most important factors were temperature of extraction and concentration of NaOH solutions. The optimum extraction conditions were A1B2C1, 4 ℃, 5% NaOH solutions and extracting only once. CONCLUSION: This optimized process is economical, simple, stable and efficient. |
| Keywords: | mussel polysaccharide orthogonal design uniform design the method of anthrone |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |